EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.31 | expressed in Escherichia coli BL21(DE3) cells | Paenibacillus sp. YM-1 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.31 | sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol 3350, 0.1 M Tris-citrate buffer, pH 6.0, 0.3 M ammonium sulfate | Paenibacillus sp. YM-1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.31 | 3.8 | - |
alpha-D-mannose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 4.2 | - |
alpha-D-glucose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 6.04 | - |
D-glucose | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.31 | 240000 | - |
gel filtration | Paenibacillus sp. YM-1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.31 | alpha-D-glucose 1-phosphate + D-glucose | Paenibacillus sp. YM-1 | - |
laminaribiose + phosphate | - |
r | |
2.4.1.31 | alpha-D-mannose 1-phosphate + D-glucose | Paenibacillus sp. YM-1 | - |
beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.31 | Paenibacillus sp. YM-1 | D7UT17 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.31 | HisTrap column chromatography and Superdex S200 gel filtration | Paenibacillus sp. YM-1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.31 | alpha-D-glucose 1-phosphate + D-glucose | - |
Paenibacillus sp. YM-1 | laminaribiose + phosphate | - |
r | |
2.4.1.31 | alpha-D-mannose 1-phosphate + D-glucose | - |
Paenibacillus sp. YM-1 | beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate | - |
r | |
2.4.1.31 | additional information | no activity with alpha-D-galactosamine 1-phosphate, alpha-D-glucosamine 1-phosphate, alpha-D-galacturonic acid 1-phosphate and alpha-D-galactose 1-phosphate | Paenibacillus sp. YM-1 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.31 | homodimer | 2 * 100000, SDS-PAGE | Paenibacillus sp. YM-1 |
2.4.1.31 | homodimer | 2 * 106000, calculated from amino acid sequence | Paenibacillus sp. YM-1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.31 | LBP | - |
Paenibacillus sp. YM-1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.31 | 0.08 | - |
alpha-D-mannose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 13 | - |
alpha-D-glucose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 15.4 | - |
D-glucose | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.31 | 0.02 | - |
alpha-D-mannose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 2.55 | - |
D-glucose | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 | |
2.4.1.31 | 3.07 | - |
alpha-D-glucose 1-phosphate | at pH 7.0 and 45°C | Paenibacillus sp. YM-1 |