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Literature summary extracted from

  • Kuhaudomlarp, S.; Walpole, S.; Stevenson, C.E.M.; Nepogodiev, S.A.; Lawson, D.M.; Angulo, J.; Field, R.A.
    Unravelling the specificity of laminaribiose phosphorylase from Paenibacillus sp. YM-1 towards donor substrates glucose/mannose 1-phosphate by using X-ray crystallography and saturation transfer difference NMR spectroscopy (2019), ChemBioChem, 20, 181-192 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.31 expressed in Escherichia coli BL21(DE3) cells Paenibacillus sp. YM-1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.31 sitting drop vapor diffusion method, using 18% (w/v) polyethylene glycol 3350, 0.1 M Tris-citrate buffer, pH 6.0, 0.3 M ammonium sulfate Paenibacillus sp. YM-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.31 3.8
-
 alpha-D-mannose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 4.2
-
 alpha-D-glucose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 6.04
-
 D-glucose at pH 7.0 and 45°C Paenibacillus sp. YM-1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.31 240000
-
 gel filtration Paenibacillus sp. YM-1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose Paenibacillus sp. YM-1
-
 laminaribiose + phosphate
-
 r
2.4.1.31 alpha-D-mannose 1-phosphate + D-glucose Paenibacillus sp. YM-1
-
 beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Paenibacillus sp. YM-1 D7UT17
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.31 HisTrap column chromatography and Superdex S200 gel filtration Paenibacillus sp. YM-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 Paenibacillus sp. YM-1 laminaribiose + phosphate
-
 r
2.4.1.31 alpha-D-mannose 1-phosphate + D-glucose
-
 Paenibacillus sp. YM-1 beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate
-
 r
2.4.1.31 additional information no activity with alpha-D-galactosamine 1-phosphate, alpha-D-glucosamine 1-phosphate, alpha-D-galacturonic acid 1-phosphate and alpha-D-galactose 1-phosphate Paenibacillus sp. YM-1 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.1.31 homodimer 2 * 100000, SDS-PAGE Paenibacillus sp. YM-1
2.4.1.31 homodimer 2 * 106000, calculated from amino acid sequence Paenibacillus sp. YM-1

Synonyms

EC Number Synonyms Comment Organism
2.4.1.31 LBP
-
 Paenibacillus sp. YM-1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.31 0.08
-
 alpha-D-mannose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 13
-
 alpha-D-glucose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 15.4
-
 D-glucose at pH 7.0 and 45°C Paenibacillus sp. YM-1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.31 0.02
-
 alpha-D-mannose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 2.55
-
 D-glucose at pH 7.0 and 45°C Paenibacillus sp. YM-1
2.4.1.31 3.07
-
 alpha-D-glucose 1-phosphate at pH 7.0 and 45°C Paenibacillus sp. YM-1