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Literature summary extracted from

  • Alam, M.S.; Moriyama, H.; Matsumoto, M.
    Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis (2019), BMC Res. Notes, 12, 163 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3) cells Dugesia ryukyuensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.004
-
 dUTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 0.0073
-
 dUTP in the presence of 5 mM Mn2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 0.0094
-
 dATP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 0.077
-
 dCTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 0.11
-
 dTTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 0.21
-
 dGTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Dugesia ryukyuensis
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Dugesia ryukyuensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 Ni-NTA column chromatography Dugesia ryukyuensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dATP + H2O
-
 Dugesia ryukyuensis dAMP + diphosphate
-
 ?
3.6.1.23 dCTP + H2O
-
 Dugesia ryukyuensis dCMP + diphosphate
-
 ?
3.6.1.23 dGTP + H2O
-
 Dugesia ryukyuensis dGMP + diphosphate
-
 ?
3.6.1.23 dTTP + H2O
-
 Dugesia ryukyuensis dTMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Dugesia ryukyuensis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O highest activity Dugesia ryukyuensis dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 homotrimer 3 * 16554, calculated from amino acid sequence Dugesia ryukyuensis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 DUT
-
 Dugesia ryukyuensis
3.6.1.23 dUTPase
-
 Dugesia ryukyuensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 1.4
-
 dTTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 1.5
-
 dATP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 1.6
-
 dCTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 2.9
-
 dGTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 34
-
 dUTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 48
-
 dUTP in the presence of 5 mM Mn2+, at pH 8.2 and 25°C Dugesia ryukyuensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.23 Dugesia ryukyuensis calculated from amino acid sequence
-
 5.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.23 13
-
 dTTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 14
-
 dGTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 16
-
 dATP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 21
-
 dCTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 6500
-
 dUTP in the presence of 5 mM Mn2+, at pH 8.2 and 25°C Dugesia ryukyuensis
3.6.1.23 8500
-
 dUTP in the presence of 5 mM Mg2+, at pH 8.2 and 25°C Dugesia ryukyuensis