Literature summary extracted from

Sa�da, F.; Uzan, M.; Lallemand, J.; Bontems, F.
New system for positive selection of recombinant plasmids and dual expression in yeast and bacteria based on the restriction ribonuclease RegB (2003), Biotechnol. Prog., 19, 727-733 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.6.1.25	by coupling the toxic restriction endoribonuclease RegB, from the bacteriophage T4, to the prokaryotic T7 and the eukaryotic GAL1 promoters, a two-function plasmid called pTOXR-1 is constructed. This plasmid is a zero-background cloning vector. It allows an efficient positive selection of recombinant plasmids without the need to completely digest, dephosphorylate, or purify the vector prior to the ligation step. The pTOXR-1 positive selection system requires no special Escherichia coli strains, no special culture media, and no addition of inducer to the selective plates. In addition, since this vector carries all signals required for both prokaryotic and eukaryotic expression, it allows the overproduction of heterologous proteins, fused to a polyhistidine tag, in the bacterium Escherichia coli and in the yeast Saccharomyces cerevisiae from a single plasmid. Hence, this vector may be a useful time-saving tool for onestep cloning and versatile protein expression in bacteria and yeast	Tequatrovirus T4

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.6.1.25	Tequatrovirus T4	P13312	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
4.6.1.25	restriction ribonuclease RegB	-	Tequatrovirus T4

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Release 2023.1 (January 2023)