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Literature summary extracted from

  • Zhao, W.; Shen, C.; Zhu, J.; Ou, C.; Liu, M.; Dai, W.; Liu, X.; Liu, J.
    Identification and characterization of methyltransferases involved in benzylisoquinoline alkaloids biosynthesis from Stephania intermedia (2019), Biotechnol. Lett., 42, 461-469 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.117 0.0536
-
 (S)-scoulerine pH 9, 37°C Stephania intermedia
2.1.1.140 0.0746
-
 (R)-coclaurine pH 9, 37°C Stephania intermedia
2.1.1.140 0.0922
-
 (R)-coclaurine pH 9, 37°C Stephania intermedia
2.1.1.140 0.0971
-
 (R)-coclaurine pH 9, 37°C Stephania intermedia

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.117 Stephania intermedia A0A5P9PAM0
-
-
2.1.1.140 Stephania intermedia A0A5P9PAN8 isoform CNMT1
-
2.1.1.140 Stephania intermedia A0A5P9PAQ9 isoform CNMT3
-
2.1.1.140 Stephania intermedia A0A5P9PAT6 isoform CNMT2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.117 additional information enzyme catalyzes O-methylation at C-9 and C-2, and O-methylation events occur first at C-9 and then at C-2 Stephania intermedia ?
-
-
2.1.1.117 S-adenosyl-L-homocysteine + (S)-tetrahydropalmatrubine
-
 Stephania intermedia S-adenosyl-L-homocysteine + (S)-tetrahydropalmatine
-
 ?
2.1.1.117 S-Adenosyl-L-methionine + (S)-scoulerine
-
 Stephania intermedia S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
 ?
2.1.1.117 S-adenosyl-L-methionine + (S)-tetrahydrocolumbamine
-
 Stephania intermedia S-adenosyl-L-homocysteine + (S)-tetrahydropalmatine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R)-coclaurine
-
 Stephania intermedia S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R)-N-methylcoclaurine
-
 Stephania intermedia S-adenosyl-L-homocysteine + (R)-magnocurarine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.117 ? x * 37670, calculated from sequence Stephania intermedia
2.1.1.140 ? x * 41080, calculated from sequence Stephania intermedia
2.1.1.140 ? x * 41600, calculated from sequence Stephania intermedia

Synonyms

EC Number Synonyms Comment Organism
2.1.1.117 SOMT
-
 Stephania intermedia
2.1.1.140 CNMT
-
 Stephania intermedia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.117 Stephania intermedia calculated from sequence
-
 5.5
2.1.1.140 Stephania intermedia calculated from sequence
-
 6
2.1.1.140 Stephania intermedia calculated from sequence
-
 6.4