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Literature summary extracted from

  • Niu, Y.F.; Wang, X.; Hu, D.X.; Balamurugan, S.; Li, D.W.; Yang, W.D.; Liu, J.S.; Li, H.Y.
    Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum (2016), Biotechnol. Biofuels, 9, 60 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 enzyme cloning and overexpression in Phaeodactylum tricornutum strain CCMP2561, PtGPAT cDNA is fused between the fucoxanthin chlorophyll a/c binding protein (fcp) fcpC promoter and fcpA terminator of Phaeodactylum tricornutum. Furthermore, an Omega leader sequence is added before the coding region of PtGPAT for enhancing its translation. A c-Myc tag is also fused to the C-terminal of PtGPAT, quantitative real-time RT-PCR expression analysis Phaeodactylum tricornutum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information enzyme overexpression in Phaeodactylum tricornutum leads to production of volumes of oil bodies and twofold increased neutral lipid content. Fatty acid composition is analyzed by GC-MS, which shows significantly higher proportion of unsaturated fatty acids compared to wild-type Phaeodactylum tricornutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast
-
 Phaeodactylum tricornutum 9507
-
2.3.1.15 additional information subcellular localization analysis, overview Phaeodactylum tricornutum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Phaeodactylum tricornutum
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Phaeodactylum tricornutum CCAP 1055/1
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Phaeodactylum tricornutum B7FRB8
-
-
2.3.1.15 Phaeodactylum tricornutum CCAP 1055/1 B7FRB8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Phaeodactylum tricornutum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Phaeodactylum tricornutum CCAP 1055/1 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Phaeodactylum tricornutum
2.3.1.15 GPAT
-
 Phaeodactylum tricornutum
2.3.1.15 gpt1
-
 Phaeodactylum tricornutum
2.3.1.15 PHATRDRAFT_42446
-
 Phaeodactylum tricornutum
2.3.1.15 PtGPAT
-
 Phaeodactylum tricornutum

General Information

EC Number General Information Comment Organism
2.3.1.15 malfunction changes in fatty acid composition regulated by overexpression of GPAT, changes in fatty acid composition in transgenic and wild-type microalgae, overview Phaeodactylum tricornutum
2.3.1.15 metabolism glycerol-3-phosphate acyltransferase (GPAT) is the critical enzyme that catalyzes the first step of triacylglycerol (TAG) formation, it is involved in the lipid metabolism of the diatom Phaeodactylum tricornutum