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Literature summary extracted from

  • Kanao, T.; Onishi, M.; Kajitani, Y.; Hashimoto, Y.; Toge, T.; Kikukawa, H.; Kamimura, K.
    Characterization of tetrathionate hydrolase from the marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH (2018), Biosci. Biotechnol. Biochem., 82, 152-160 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.12.1.B1 expression in Escherichia coli BL21 StarTM(DE3) Acidithiobacillus thiooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.12.1.B1 KNO3 1 M, 40% loss of activity Acidithiobacillus thiooxidans
3.12.1.B1 NaNO3 1 M, 20% loss of activity Acidithiobacillus thiooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.12.1.B1 outer membrane localized on the outer membrane of the cell, and acts as a peripheral protein Acidithiobacillus thiooxidans 19867
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.12.1.B1 (NH4)2SO4 1 M, 4fold increase in activity Acidithiobacillus thiooxidans
3.12.1.B1 KCl 2 M, 2.5fold increase in activity Acidithiobacillus thiooxidans
3.12.1.B1 Na2SO4 1 M, 2.5fold increase in activity Acidithiobacillus thiooxidans
3.12.1.B1 NaCl 1 M, 2.5fold increase in activity Acidithiobacillus thiooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.12.1.B1 97000
-
 gel filtration Acidithiobacillus thiooxidans

Organism

EC Number Organism UniProt Comment Textmining
3.12.1.B1 Acidithiobacillus thiooxidans
-
-
-
3.12.1.B1 Acidithiobacillus thiooxidans SH
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.12.1.B1 partial Acidithiobacillus thiooxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.12.1.B1 culture condition:sulfur-grown cell
-
 Acidithiobacillus thiooxidans
-
3.12.1.B1 culture condition:tetrathionate-grown cell
-
 Acidithiobacillus thiooxidans
-

Subunits

EC Number Subunits Comment Organism
3.12.1.B1 homodimer 2 * 52000, SDS-PAGE Acidithiobacillus thiooxidans

Synonyms

EC Number Synonyms Comment Organism
3.12.1.B1 4THase
-
 Acidithiobacillus thiooxidans
3.12.1.B1 SH-tth
-
 Acidithiobacillus thiooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.12.1.B1 55
-
-
 Acidithiobacillus thiooxidans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.12.1.B1 20 65 20°C: about 40% of maximal activity, 65°C: about 65% of maximal activity Acidithiobacillus thiooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.12.1.B1 3
-
-
 Acidithiobacillus thiooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.12.1.B1 1 4 pH 1.0: about 40% of maximal activity, pH 4.0: about 55% of maximal activity Acidithiobacillus thiooxidans

General Information

EC Number General Information Comment Organism
3.12.1.B1 metabolism the enzyme is involved in tetrathionate metabolism, but also in thiosulfate and elemental sulfur metabolism Acidithiobacillus thiooxidans