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Literature summary extracted from

  • Venkateswar Reddy, M.; Mawatari, Y.; Onodera, R.; Nakamura, Y.; Yajima, Y.; Chang, Y.
    Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii (2017), Biores. Technol., 234, 99-105 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.304 synthesis replacement of conventional plastics by bioplastics. Polyhydroxyalkanoates (PHA) are bio-polyesters accumulated in cells by a wide range of bacteria. Polyhydroxyalkanoates production from synthetic waste using Pseudomonas palleronii polyhydroxyalkanoate synthase enzyme activity Hydrogenophaga palleronii
2.3.1.304 synthesis replacement of conventional plastics by bioplastics. Polyhydroxyalkanoates (PHA) are bio-polyesters accumulated in cells by a wide range of bacteria. Polyhydroxyalkanoates production from synthetic waste using Pseudomonas pseudoflava polyhydroxyalkanoate synthase enzyme activity Hydrogenophaga pseudoflava
2.3.1.304 synthesis the enzyme can be used for polyhydroxyalkanoate production from synthetic wastewater Hydrogenophaga pseudoflava

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n Hydrogenophaga palleronii
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n Hydrogenophaga palleronii
-
 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n Hydrogenophaga pseudoflava
-
 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n Hydrogenophaga pseudoflava NBRC-102513
-
 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 additional information Hydrogenophaga palleronii the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile atty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview ?
-
-
2.3.1.304 additional information Hydrogenophaga pseudoflava the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity ?
-
-
2.3.1.304 additional information Hydrogenophaga pseudoflava NBRC-102513 the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Hydrogenophaga palleronii
-
-
-
2.3.1.304 Hydrogenophaga pseudoflava
-
-
-
2.3.1.304 Hydrogenophaga pseudoflava A0A4P6X2C3
-
-
2.3.1.304 Hydrogenophaga pseudoflava NBRC-102513
-
-
-
2.3.1.304 Hydrogenophaga pseudoflava NBRC-102513 A0A4P6X2C3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 Hydrogenophaga palleronii CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n
-
 Hydrogenophaga pseudoflava CoA + poly[(R)-3-hydroxybutyrate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n
-
 Hydrogenophaga palleronii CoA + poly[(R)-3-hydroxybutyrate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxybutyrate]n
-
 Hydrogenophaga pseudoflava NBRC-102513 CoA + poly[(R)-3-hydroxybutyrate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate]
-
 Hydrogenophaga pseudoflava CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate]
-
 Hydrogenophaga palleronii CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate]
-
 Hydrogenophaga pseudoflava NBRC-102513 CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate]
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n
-
 Hydrogenophaga palleronii [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n
-
 Hydrogenophaga pseudoflava [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 (R)-3-hydroxybutanoyl-CoA + [(R)-3-hydroxybutanoate]n
-
 Hydrogenophaga pseudoflava NBRC-102513 [(R)-3-hydroxybutanoate]n+1 + CoA
-
 ?
2.3.1.304 additional information the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile atty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview Hydrogenophaga palleronii ?
-
-
2.3.1.304 additional information the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity Hydrogenophaga pseudoflava ?
-
-
2.3.1.304 additional information the bacteria produce homo-polymer [poly-3-hydroxybutyrate (P3HB)] when only acetate is used as carbon source, and it produces co-polymer [poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) P(3HB-co-3HV)] by addition of co-substrate propionate. Evaluation of PHA production by Pseudomonas pseudoflava strain NBRC-102513 from wastewater containing diverse volatile fatty acids (VFA), common products of various wastewaters. Analysis of the PHA spectrum produced from different carbon sources, NMR study, overview. MW and polydispersity index (PDI, Mw/Mn) of the P3HB produced by Pseudomonas pseudoflava is 17.63 kDa and 3.3 respectively. MW and PDI of the co-polymer P(3HB-co-3HV) produced by Pseudomonas pseudoflava is 52.33 kDa and 5.7 respectively. The Mw of the standard P(3HB-co-3HV) is 110 kDa, and PDI is 4.3. Pseudomonas pseudoflava can produce biopolymers with relatively lower dispersity Hydrogenophaga pseudoflava NBRC-102513 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.3.1.304 ? x * 63000, SDS-PAGE Hydrogenophaga pseudoflava
2.3.1.304 ? x * 63000, SDS-PAGE Hydrogenophaga palleronii

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 PHA synthase
-
 Hydrogenophaga pseudoflava
2.3.1.304 PHA synthase
-
 Hydrogenophaga palleronii
2.3.1.304 polyhydroxyalkanoate synthase
-
 Hydrogenophaga palleronii
2.3.1.304 polyhydroxyalkanoate synthase
-
 Hydrogenophaga pseudoflava

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.304 30
-
 in vivo assay at Hydrogenophaga palleronii
2.3.1.304 30
-
 in vivo assay at Hydrogenophaga pseudoflava

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.304 7 9.2 in vivo activity Hydrogenophaga pseudoflava

General Information

EC Number General Information Comment Organism
2.3.1.304 evolution PHA synthases have been assigned to three classes based on their substrate specificity and subunit composition. The class I PHA synthases are composed of a single type of polypeptide chain and use mainly short chain length hydroxyalkanoic acid CoA thioesters as substrates. The class III PHA synthases are composed by two different subunits, each of approximately 40 kDa. Substrate specificity is the main difference between class II and both class I and class III PHA synthases. Class II PHA synthases integrate specially 3-hydroxyfatty acids of medium chain length (C6-C14) into PHA, and the resulting product is a latex-like polymer. Class I PHA synthases synthesize higher molecular weight PHAs compared with class II PHA synthases Hydrogenophaga palleronii
2.3.1.304 evolution PHA synthases have been assigned to three classes based on their substrate specificity and subunit composition. The class I PHA synthases are composed of a single type of polypeptide chain and use mainly short chain length hydroxyalkanoic acid CoA thioesters as substrates. The class III PHA synthases are composed by two different subunits, each of approximately 40 kDa. Substrate specificity is the main difference between class II and both class I and class III PHA synthases. Class II PHA synthases integrate specially 3-hydroxyfatty acids of medium chain length (C6-C14) into PHA, and the resulting product is a latex-like polymer. Class I PHA synthases synthesize higher molecular weight PHAs compared with class II PHA synthases Hydrogenophaga pseudoflava
2.3.1.304 metabolism PHA synthase is the critical enzyme in PHA biosynthesis Hydrogenophaga palleronii
2.3.1.304 metabolism PHA synthase is the critical enzyme in PHA biosynthesis Hydrogenophaga pseudoflava
2.3.1.304 physiological function PHA synthase is the critical enzyme in polyhydroxyalkanoates (PHA) biosynthesis, and R-3-hydroxyacyl-coenzyme A (CoA) is the substrate. Poly-3-hydroxybutyrate (P3HB) is one type of PHA produced by many bacteria Hydrogenophaga palleronii
2.3.1.304 physiological function PHA synthase is the critical enzyme in polyhydroxyalkanoates (PHA) biosynthesis, and R-3-hydroxyacyl-coenzyme A (CoA) is the substrate. Poly-3-hydroxybutyrate (P3HB) is one type of PHA produced by many bacteria Hydrogenophaga pseudoflava