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Literature summary extracted from

  • Huo, Y.; Zhan, Y.; Wang, Q.; Li, S.; Yang, S.; Nomura, C.T.; Wang, C.; Chen, S.
    Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02 enzymatic properties and efficient functions for acetoin/butanediol and L-valine biosynthesis (2018), Bioprocess Biosyst. Eng., 41, 87-96 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 L-isoleucine
-
 Bacillus licheniformis
2.2.1.6 L-leucine
-
 Bacillus licheniformis
2.2.1.6 L-valine
-
 Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 3.96
-
 pyruvate at pH 6.5 and 37°C Bacillus licheniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Bacillus licheniformis
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate Bacillus licheniformis WX-02
-
 2-acetolactate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Bacillus licheniformis
-
-
-
2.2.1.6 Bacillus licheniformis WX-02
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6 Ni-NTA column chromatography Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 35.4
-
 at pH 6.5 and 37°C Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Bacillus licheniformis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Bacillus licheniformis WX-02 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 63000, SDS-PAGE Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetolactate synthase
-
 Bacillus licheniformis
2.2.1.6 AlsS
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
 at pH 6.5 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.6 20 50 the enzyme shows more than 50% activity between 20 and 50°C. The activity at 60°C is less than 40% and then completely decreases at 70-80°C Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 514
-
 pyruvate at pH 6.5 and 37°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 6.5
-
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.6 6 7.5 the enzyme exhibits more than 60% activity in the range of pH 5.5-7.5 Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate
-
 Bacillus licheniformis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 0.00273
-
 L-valine at pH 6.5 and 37°C Bacillus licheniformis
2.2.1.6 0.0119
-
 L-leucine at pH 6.5 and 37°C Bacillus licheniformis
2.2.1.6 0.19
-
 L-isoleucine at pH 6.5 and 37°C Bacillus licheniformis

General Information

EC Number General Information Comment Organism
2.2.1.6 malfunction the enzyme-deficient strain cannot produce acetoin, 2,3-butanediol, and L-valine Bacillus licheniformis
2.2.1.6 metabolism enzyme overexpression can effectively improve acetoin/2,3-butanediol and L-valine production in Bacillus licheniformis Bacillus licheniformis