EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.5.7 | industry | under optimized conditions, using the fed-batch reaction mode, total of 1050 mM 3-cyanopyridine is hydrolyzed completely in 20.8 h with eight substrate feedings, yielding 129.2 g/l production of nicotinic acid and thus showing a potential for industrial application | Cupriavidus necator |
3.5.5.7 | synthesis | under optimized conditions, using the fed-batch reaction mode, total of 1050 mM 3-cyanopyridine is hydrolyzed completely in 20.8 h with eight substrate feedings, yielding 129.2 g/l production of nicotinic acid and thus showing a potential for industrial application | Cupriavidus necator |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.5.7 | overexpressed in Escherichia coli BL21(DE3) | Cupriavidus necator |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.7 | acetone | 25% (v/v), about 40% inhibition | Cupriavidus necator | |
3.5.5.7 | Ag+ | 5 mM, 94% inhibition | Cupriavidus necator | |
3.5.5.7 | Ca2+ | 5 mM, 97% inhibition | Cupriavidus necator | |
3.5.5.7 | Co2+ | 5 mM, 15% inhibition | Cupriavidus necator | |
3.5.5.7 | Cu2+ | 5 mM, 70% inhibition | Cupriavidus necator | |
3.5.5.7 | dimethyl sulfoxide | 15% (v/v),about 35% inhibition | Cupriavidus necator | |
3.5.5.7 | ethanol | 25% (v/v), about 20% inhibition | Cupriavidus necator | |
3.5.5.7 | Fe2+ | 5 mM, 51% inhibition | Cupriavidus necator | |
3.5.5.7 | Isopropanol | 25% (v/v), about 10% inhibition | Cupriavidus necator | |
3.5.5.7 | Li+ | 5 mM, 37% inhibition | Cupriavidus necator | |
3.5.5.7 | methanol | 25% (v/v), about 20% inhibition | Cupriavidus necator | |
3.5.5.7 | Mg2+ | 5 mM, 31% inhibition | Cupriavidus necator | |
3.5.5.7 | Mn2+ | 5 mM, 61% inhibition | Cupriavidus necator | |
3.5.5.7 | Ni2+ | 5 mM, 47% inhibition | Cupriavidus necator | |
3.5.5.7 | tetrahydrofuran | 10% (v/v), about 95% inhibition | Cupriavidus necator | |
3.5.5.7 | Zn2+ | 5 mM, 47% inhibition | Cupriavidus necator |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.7 | 0.4 | - |
3-Cyanopyridine | pH 7.0, 30°C | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.7 | Cupriavidus necator | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.7 | 2-cyanopyridine + 2 H2O | activity is 3.35fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | pyridine 2-carboxylic acid + NH3 | - |
? | |
3.5.5.7 | 2-methylglutaronitrile + 2 H2O | activity is 10% compared to activity with 3-cyanopyridine | Cupriavidus necator | 4-cyanopentanoic acid + NH3 | - |
? | |
3.5.5.7 | 2-phenylbutyronitrile + 2 H2O | activity is 11% compared to activity with 3-cyanopyridine | Cupriavidus necator | 2-phenylpropionic acid + NH3 | - |
? | |
3.5.5.7 | 3-cyanopyridine + 2 H2O | - |
Cupriavidus necator | pyridine 3-carboxylic acid + NH3 | - |
? | |
3.5.5.7 | 3-hydroxyglutaronitrile + 4 H2O | activity is 38% compared to activity with 3-cyanopyridine | Cupriavidus necator | 3-hydroxyglutaric acid + 2 NH3 | - |
? | |
3.5.5.7 | 3-hydroxypropionitrile + 2 H2O | activity is 1.5fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | 3-hydroxypropionitrile + NH3 | - |
? | |
3.5.5.7 | 3-phenylpropionitrile + 2 H2O | activity is 61% compared to activity with 3-cyanopyridine | Cupriavidus necator | 3-phenylpropionic acid + NH3 | - |
? | |
3.5.5.7 | 4-chlorobutyronitrile + 2 H2O | activity is 1.49fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | 4-chlorobutyric acid + NH3 | - |
? | |
3.5.5.7 | 4-cyanopyridine + 2 H2O | activity is 3fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | pyridine 4-carboxylic acid + NH3 | - |
? | |
3.5.5.7 | adiponitrile + 4 H2O | activity is 1.14fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | adipic acid + 2 NH3 | - |
? | |
3.5.5.7 | cinnamonitrile + 2 H2O | activity is 8fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | cinnamic acid + NH3 | - |
? | |
3.5.5.7 | fumaronitrile + 4 H2O | activity is 36fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | fumaric acid + 2 NH3 | - |
? | |
3.5.5.7 | glycolonitrile + 2 H2O | activity is 15.9fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | glycolic acid + NH3 | - |
? | |
3.5.5.7 | iminodiacetonitrile + 4 H2O | activity is 39% compared to activity with 3-cyanopyridine | Cupriavidus necator | iminodiacetic acid + 2 NH3 | - |
? | |
3.5.5.7 | indole-3-acetonitrile + 2 H2O | activity is 48% compared to activity with 3-cyanopyridine | Cupriavidus necator | indol-3-acetic acid + NH3 | - |
? | |
3.5.5.7 | additional information | the enzyme shows a broad hydrolytic activity toward aliphatic and heterocyclic nitriles and shows high tolerance of 3-cyanopyridine. No activity with: benzonitrile, phenylacetonitrile, 4-chlorobenzyl cyanide, mandelonitrile, 2-chloromandelonitrile, alpha-methylphenylacetonitrile, 1,2-phenylenediacetonitrile, dodecanenitrile | Cupriavidus necator | ? | - |
- |
|
3.5.5.7 | pimelonitrile + 4 H2O | activity is 85% compared to activity with 3-cyanopyridine | Cupriavidus necator | pimelic acid + 2 NH3 | - |
? | |
3.5.5.7 | sebaconitrile + 4 H2O | activity is 1.66fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | sebaconic acid + 2 NH3 | - |
? | |
3.5.5.7 | succinonitrile + 4 H2O | activity is 1.97fold higher compared to activity with 3-cyanopyridine | Cupriavidus necator | succinic acid + 2 NH3 | - |
? | |
3.5.5.7 | valeronitrile + 2 H2O | activity is 99% compared to activity with 3-cyanopyridine | Cupriavidus necator | valeric acid + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.5.7 | REH16 | - |
Cupriavidus necator |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 37 | - |
- |
Cupriavidus necator |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 20 | 50 | 20°C: about 60% of maximal activity, 50°C: about 85% of maximal activity | Cupriavidus necator |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 25 | - |
120 h, 5% loss of activity | Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 6.6 | - |
- |
Cupriavidus necator |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.7 | 5 | 8.5 | pH 5.0: about 40% of maximal activity, pH 8.5: about 55% of maximal activity | Cupriavidus necator |