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Literature summary extracted from

  • Wilson, W.A.; Pradhan, P.; Madhan, N.; Gist, G.C.; Brittingham, A.
    Glycogen synthase from the parabasalian parasite Trichomonas vaginalis An unusual member of the starch/glycogen synthase family (2017), Biochimie, 138, 90-101 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.11 expressed in Escherichia coli strain BL21(DE3) and in Saccharomyces cerevisiae strain WW100 lacking endogenous glycogen synthase activity Trichomonas vaginalis
2.4.1.21 expressed in Escherichia coli strain BL21(DE3) and in Saccharomyces cerevisiae strain WW100 lacking endogenous glycogen synthase activity Trichomonas vaginalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.11 0.056
-
 UDP-glucose at pH 7.8, temperature not specified in the publication Trichomonas vaginalis
2.4.1.21 0.058
-
 ADP-alpha-D-glucose at pH 7.8, temperature not specified in the publication Trichomonas vaginalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.11 UDP-glucose + glycogen Trichomonas vaginalis
-
 UDP + ?
-
 ?
2.4.1.11 UDP-glucose + glycogen Trichomonas vaginalis ATCC PRA-98
-
 UDP + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.11 Trichomonas vaginalis
-
-
-
2.4.1.11 Trichomonas vaginalis ATCC PRA-98
-
-
-
2.4.1.21 Trichomonas vaginalis
-
-
-
2.4.1.21 Trichomonas vaginalis ATCC PRA-98
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.11 Bio-Scale Mini Profinity IMAC Trichomonas vaginalis
2.4.1.21 Bio-Scale Mini Profinity IMAC Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.11 2.5
-
 at pH 7.8, temperature not specified in the publication Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.11 additional information the enzyme is not able to transfer glucose from UDP-glucose to glucose itself Trichomonas vaginalis ?
-
-
2.4.1.11 additional information the enzyme is not able to transfer glucose from UDP-glucose to glucose itself Trichomonas vaginalis ATCC PRA-98 ?
-
-
2.4.1.11 UDP-glucose + glycogen
-
 Trichomonas vaginalis UDP + ?
-
 ?
2.4.1.11 UDP-glucose + glycogen
-
 Trichomonas vaginalis ATCC PRA-98 UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltoheptaose
-
 Trichomonas vaginalis UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltoheptaose
-
 Trichomonas vaginalis ATCC PRA-98 UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltose
-
 Trichomonas vaginalis UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltose
-
 Trichomonas vaginalis ATCC PRA-98 UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltotetraose
-
 Trichomonas vaginalis UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltotetraose
-
 Trichomonas vaginalis ATCC PRA-98 UDP + ?
-
 ?
2.4.1.11 UDP-glucose + maltotriose
-
 Trichomonas vaginalis UDP + ?
-
 ?
2.4.1.21 ADP-alpha-D-glucose + glycogen
-
 Trichomonas vaginalis ADP + ?
-
 ?
2.4.1.21 ADP-alpha-D-glucose + maltose
-
 Trichomonas vaginalis ADP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.11 ? x * 56000, SDS-PAGE Trichomonas vaginalis
2.4.1.21 ? x * 56000, SDS-PAGE Trichomonas vaginalis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.11 glycogen synthase
-
 Trichomonas vaginalis
2.4.1.11 starch/glycogen synthase
-
 Trichomonas vaginalis
2.4.1.11 TVAG_258220 open reading frame Trichomonas vaginalis
2.4.1.21 starch/glycogen synthase
-
 Trichomonas vaginalis
2.4.1.21 TVAG_258220 open reading frame Trichomonas vaginalis