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Literature summary extracted from

  • McFie, P.J.; Izzard, S.; Vu, H.; Jin, Y.; Beauchamp, E.; Berthiaume, L.G.; Stone, S.J.
    Membrane topology of human monoacylglycerol acyltransferase-2 and identification of regions important for its localization to the endoplasmic reticulum (2016), Biochim. Biophys. Acta, 1861, 1192-1204 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.22 expression in HEK-293T cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.22 C122A about 20-30% of wild-type activity Homo sapiens
2.3.1.22 C313A about 20-30% of wild-type activity Homo sapiens
2.3.1.22 C334A located at the extreme C-terminus, about 200% of wild-type activity Homo sapiens
2.3.1.22 C34A about 20-30% of wild-type activity Homo sapiens
2.3.1.22 DELTAC76 about 20-30% of wild-type activity Homo sapiens
2.3.1.22 additional information a mutant lacking all five cysteine residues is devoid of monoacylglycerol acyltransferase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.22 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Homo sapiens
2.3.1.22 N-ethylmaleimide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.22 endoplasmic reticulum integral membrane protein, the C-terminus is exposed to the cytosol, while the N-terminus is partially buried in the ER membrane. Domains that are important for catalysis face the cytosol. Both transmembrane domains of MGAT2 are important for its ER localization, and MGAT2 is present in mitochondrial-associated membranes Homo sapiens 5783
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.22 Homo sapiens Q3SYC2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.22 [N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)-methyl]amino]palmitoyl-CoA + 2-oleoylglycerol
-
 Homo sapiens CoA + 1-[N-[(7-nitro-2-1,3-benzoxadiazol-4-yl)-methyl]amino]palmitoyl-2-oleoylglycerol
-
-