EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.90 | expressed in Escherichia coli BL21(DE3) cells | Leisingera caerulea |
1.14.11.72 | expressed in Escherichia coli BL21(DE3) cells | Leisingera caerulea |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.90 | in complex with Fe2+ and [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate, hanging drop vapor diffusion method, using 0.2 M CaCl2, 0.1 M sodium HEPES (pH 7.5), and 27-33% polyethylene glycol 4000 | Leisingera caerulea |
1.14.11.72 | in complex with Fe2+, [2-(trimethylamino)ethyl]phosphonate and 2-oxoglutarate, hanging drop vapor diffusion method, using 1.0-1.4 M Li2SO4 and 1.6-1.8 M (NH4)2SO4 | Leisingera caerulea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.78 | Fe2+ | dependent on | uncultured bacterium HF130_AEPn_1 | |
1.13.11.90 | Iron | the enzyme uses a mixed-valent diiron cofactor | Leisingera caerulea | |
1.14.11.72 | Fe2+ | dependent on | Leisingera caerulea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.72 | 70000 | - |
gel filtration | Leisingera caerulea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.78 | (2-amino-1-hydroxyethyl)phosphonate + O2 | uncultured bacterium HF130_AEPn_1 | - |
glycine + phosphate | - |
? | |
1.13.11.90 | [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2 | Leisingera caerulea | - |
glycine betaine + phosphate | - |
? | |
1.14.11.72 | [2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 | Leisingera caerulea | - |
[(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.78 | uncultured bacterium HF130_AEPn_1 | D0E8I5 | - |
- |
1.13.11.90 | Leisingera caerulea | A0A4V8H040 | - |
- |
1.14.11.72 | Leisingera caerulea | A0A4V8H042 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.90 | Ni-NTA column chromatography and Sephadex S-200 gel filtration | Leisingera caerulea |
1.14.11.72 | Ni-NTA column chromatography and Sephadex S-200 gel filtration | Leisingera caerulea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.78 | (2-amino-1-hydroxyethyl)phosphonate + O2 | - |
uncultured bacterium HF130_AEPn_1 | glycine + phosphate | - |
? | |
1.13.11.90 | [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + O2 | - |
Leisingera caerulea | glycine betaine + phosphate | - |
? | |
1.14.11.72 | 2-aminoethylphosphonate + 2-oxoglutarate + O2 | - |
Leisingera caerulea | ? | - |
? | |
1.14.11.72 | 3-aminopropylphosphonate + 2-oxoglutarate + O2 | - |
Leisingera caerulea | ? | - |
? | |
1.14.11.72 | additional information | no activity with gamma-butyrobetaine or analogue substrates | Leisingera caerulea | ? | - |
- |
|
1.14.11.72 | phosphocholine + 2-oxoglutarate + O2 | - |
Leisingera caerulea | glycine betaine aldehyde + phosphate + succinate + CO2 | - |
? | |
1.14.11.72 | [2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 | - |
Leisingera caerulea | [(1R)-1-hydroxy-2-(dimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 | - |
? | |
1.14.11.72 | [2-(trimethylamino)ethyl]phosphonate + 2-oxoglutarate + O2 | - |
Leisingera caerulea | [(1R)-1-hydroxy-2-(trimethylamino)ethyl]phosphonate + succinate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.11.72 | homodimer | 2 * 41000, calculated from amino acid sequence | Leisingera caerulea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.78 | (R)-1-hydroxy-2-aminoethylphosphonate oxygenase | - |
uncultured bacterium HF130_AEPn_1 |
1.13.11.78 | phnZ | - |
uncultured bacterium HF130_AEPn_1 |
1.13.11.90 | tmpB | - |
Leisingera caerulea |
1.14.11.72 | gamma-butyrobetaine hydroxylase | misannotation | Leisingera caerulea |
1.14.11.72 | tmpA | - |
Leisingera caerulea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.72 | 2-oxoglutarate | dependent on | Leisingera caerulea |