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Literature summary extracted from

  • Garcia-Fabiani, M.B.; Montanaro, M.A.; Stringa, P.; Lacunza, E.; Cattaneo, E.R.; Santana, M.; Pellon-Maison, M.; Gonzalez-Baro, M.R.
    Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis (2017), Biochem. J., 474, 3093-3107 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene GPAT2 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information the gene is silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting Gpat2 mRNA into mouse testis, expression of 5 different shRNA-Gpat2 vectors, subcloning in HEK-293 cells. Histological and gene expression analysis shows impaired spermatogenesis and arrest at the pachytene stage. Defects in reproductive fitness are also observed, and the analysis of apoptosis-related gene expression demonstrates the activation of apoptosis in Gpat2-silenced germ cells. The decrease in germ cell number in shRNA-Gpat2 mice seminiferous tubules correlates with apoptosis activation, without retrotransposon derepression Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Mus musculus
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 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Mus musculus Q14DK4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 carcinoma cell
-
 Mus musculus
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2.3.1.15 germ cell
-
 Mus musculus
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2.3.1.15 additional information the GPAT2 isoform expression is restricted to male germ cells and cancer cells Mus musculus
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2.3.1.15 testis GPAT2 expression in mouse testis fluctuates during sexual maturation, Gpat2 expression in mouse testis is maximal at the pachytene stage Mus musculus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase 2
-
 Mus musculus
2.3.1.15 GPAT2
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.15 malfunction the gene is silenced in vivo by inoculating lentiviral particles carrying the sequence of a short-hairpin RNA targeting Gpat2 mRNA into mouse testis. Histological and gene expression analysis shows impaired spermatogenesis and arrest at the pachytene stage. The enzyme deficiency triggers apoptotic mechanisms and affects reproductive capacity Mus musculus
2.3.1.15 physiological function glycerol-3-phosphate acyltransferases (GPAT) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. Glycerol-3-phosphate acyltransferase 2 is essential for normal spermatogenesis. GPAT is regulated by epigenetic mechanisms in combination with vitamin A derivatives, analysis of GPAT2 role in the developing male germ cells, overview. GPAT2 protein is necessary for the normal development of male gonocytes. GPAT2 is necessary to reach or to complete the pachytene stage in which GPAT2 interacts with MILI to synthesize piRNAs by the primary pathway Mus musculus