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Literature summary extracted from

  • Piano, V.; Nenci, S.; Magnani, F.; Aliverti, A.; Mattevi, A.
    Recombinant human dihydroxyacetonephosphate acyl-transferase characterization as an integral monotopic membrane protein (2016), Biochem. Biophys. Res. Commun., 481, 51-58 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.42 expressed in HEK-293E cells and Pichia pastoris KM71H MutS strain Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.42 DHAPATDELTA135 the mutant enzyme is completely inactive Homo sapiens

General Stability

EC Number General Stability Organism
2.3.1.42 the enzyme activity and stability is dependent on alkyl-dihydroxyacetone phosphate synthase presence Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 peroxisomal membrane inner side of the peroxisomal membrane Homo sapiens 5778
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.42 acyl-CoA + glycerone phosphate Homo sapiens
-
 CoA + acylglycerone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Homo sapiens O15228
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.42 Ni-Sepharose resin column chromatography, His-Trap column chromatography and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + glycerone phosphate
-
 Homo sapiens CoA + acylglycerone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.42 ? x * 70000, recombinant enzyme, SDS-PAGE Homo sapiens
2.3.1.42 ? x * 109000, recombinant enzyme fused to enhanced green fluorescent protein, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.42 DHAPAT
-
 Homo sapiens
2.3.1.42 dihydroxyacetone phosphate acyl-transferase
-
 Homo sapiens