EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.93 | recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.93 | additional information | the purified recombinant His-tagged enzyme is immobilized on different supports, i.e. an epoxy-activated support LX1000-EPC4 (EP) or its derivatives, EP-polyethyleneimine (EP-PEI) and EP-ethylenediamine (EP-EDA) with cationic groups on the surface, validation and optimization. The CCA immobilized on EP-PEI has the best stability and its activity declines very slowly at 45°C. The enzyme immobilized on the cationic support EP-EDA has a half-life of 100 min at 45°C and pH 8.0, a value much lower than on EP-PEI. Stabilization factors of enzyme immobilized on EP-PEI and EP-EDA are 35.6fold and 5.5fold, respectively. The Km values of CCA immobilized on EP-PEI and EP-EDA are even lower than that of the free enzyme, indicating that a higher substrate affinity is obtained after the immobilization on the cationic supports. The reusability of the EP-PEI support significantly reduces the cost of the biocatalyst preparation | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.93 | additional information | - |
additional information | Michaelis constant (Km) and maximum reaction velocity (Vmax) for the free and immobilized enzyme are calculated according to Lineweaver-Burk plots | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.93 | cephalosporin C + H2O | Pseudomonas sp. | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.93 | Pseudomonas sp. | A0A1D8GRD5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.93 | cephalosporin C + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
3.5.1.93 | additional information | substrate chephalosporin has a similar negative charge in pH 8.5 solution | Pseudomonas sp. | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.93 | CCA | - |
Pseudomonas sp. |
3.5.1.93 | cephalosporin C acylase | - |
Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.93 | 37 | - |
- |
Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.93 | 8.5 | - |
- |
Pseudomonas sp. |