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Literature summary extracted from

  • Sharma, R.; Rani, C.; Mehra, R.; Nargotra, A.; Chib, R.; Rajput, V.; Kumar, S.; Singh, S.; Sharma, P.; Khan, I.
    Identification and characterization of novel small molecule inhibitors of the acetyltransferase activity of Escherichia coli N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase (GlmU) (2016), Appl. Microbiol. Biotechnol., 100, 3071-3085 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.157 expression in Escherichia coli Haemophilus influenzae
2.3.1.157 expression in Escherichia coli Escherichia coli
2.7.7.23 expression in Escherichia coli Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.157 (5-[(E)-[(2,5-dimethylphenyl)imino]methyl]furan-2-yl)(hydroxy)oxoammonium 0.1 mM, 93.82% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 (5-[(E)-[(2,5-dimethylphenyl)imino]methyl]furan-2-yl)(hydroxy)oxoammonium
-
 Haemophilus influenzae
2.3.1.157 3-fluoro-N-[1-(2-methylpropanoyl)-1,2,3,4-tetrahydroquinolin-6-yl]benzene-1-sulfonamide 0.1 mM, 82.87% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 3-fluoro-N-[1-(2-methylpropanoyl)-1,2,3,4-tetrahydroquinolin-6-yl]benzene-1-sulfonamide
-
 Haemophilus influenzae
2.3.1.157 3-hydrazinylquinoline-2-thiol 0.1 mM, 92.68% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 3-hydrazinylquinoline-2-thiol
-
 Haemophilus influenzae
2.3.1.157 5,7-dichloro-2-hydrazinylquinolin-8-ol 0.1 mM, 98.25% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 5,7-dichloro-2-hydrazinylquinolin-8-ol
-
 Haemophilus influenzae
2.3.1.157 6-chloro-N-[3-(methylsulfanyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-7-sulfonamide 0.1 mM, 68.69% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 6-chloro-N-[3-(methylsulfanyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-7-sulfonamide
-
 Haemophilus influenzae
2.3.1.157 N-[2,4-dimethoxy-5-(10H-phenoxazine-10-sulfonyl)phenyl]-2-(pyridin-4-yl)acetamide
-
 Escherichia coli
2.3.1.157 N-[2,4-dimethoxy-5-(10H-phenoxazine-10-sulfonyl)phenyl]-2-(pyridin-4-yl)acetamide
-
 Haemophilus influenzae
2.3.1.157 [4-fluoro-3-[(E)-([5-[hydroxy(oxo)azaniumyl]furan-2-yl]methylidene)amino]phenyl](hydroxy)oxoammonium 0.1 mM, 97.47% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 [4-fluoro-3-[(E)-([5-[hydroxy(oxo)azaniumyl]furan-2-yl]methylidene)amino]phenyl](hydroxy)oxoammonium
-
 Haemophilus influenzae
2.3.1.157 [5-[(E)-2-(1-butyl-1H-benzimidazol-2-yl)ethenyl]furan-2-yl](hydroxy)oxoammonium 0.1 mM, 84.26% inhibition, competitive inhibition versus acetyl-CoA, uncompetitive inhibition versus glucose 1-phosphate Escherichia coli
2.3.1.157 [5-[(E)-2-(1-butyl-1H-benzimidazol-2-yl)ethenyl]furan-2-yl](hydroxy)oxoammonium
-
 Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Haemophilus influenzae the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Escherichia coli the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Escherichia coli ATCC 25922 the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Escherichia coli the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Escherichia coli ATCC 25922 the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Escherichia coli
-
-
-
2.3.1.157 Escherichia coli ATCC 25922
-
-
-
2.3.1.157 Haemophilus influenzae
-
-
-
2.7.7.23 Escherichia coli
-
-
-
2.7.7.23 Escherichia coli ATCC 25922
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157
-
 Haemophilus influenzae
2.3.1.157
-
 Escherichia coli
2.7.7.23
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Haemophilus influenzae CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Escherichia coli CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Haemophilus influenzae CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Escherichia coli ATCC 25922 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli ATCC 25922 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Escherichia coli diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Escherichia coli ATCC 25922 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli ATCC 25922 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 GlmU
-
 Haemophilus influenzae
2.3.1.157 GlmU
-
 Escherichia coli
2.3.1.157 N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase
-
 Haemophilus influenzae
2.3.1.157 N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase
-
 Escherichia coli
2.7.7.23 GlmU
-
 Escherichia coli
2.7.7.23 N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.23 30
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.23 7.6
-
 assay at Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.157 0.00054
-
 pH 7.3, 37°C Escherichia coli N-[2,4-dimethoxy-5-(10H-phenoxazine-10-sulfonyl)phenyl]-2-(pyridin-4-yl)acetamide
2.3.1.157 0.0014
-
 pH 7.3, 37°C Escherichia coli 5,7-dichloro-2-hydrazinylquinolin-8-ol
2.3.1.157 0.00376
-
 pH 7.3, 37°C Escherichia coli 3-hydrazinylquinoline-2-thiol
2.3.1.157 0.00405
-
 pH 7.3, 37°C Escherichia coli [4-fluoro-3-[(E)-([5-[hydroxy(oxo)azaniumyl]furan-2-yl]methylidene)amino]phenyl](hydroxy)oxoammonium
2.3.1.157 0.02105
-
 pH 7.3, 37°C Escherichia coli (5-[(E)-[(2,5-dimethylphenyl)imino]methyl]furan-2-yl)(hydroxy)oxoammonium
2.3.1.157 0.0249
-
 pH 7.3, 37°C Escherichia coli 3-fluoro-N-[1-(2-methylpropanoyl)-1,2,3,4-tetrahydroquinolin-6-yl]benzene-1-sulfonamide
2.3.1.157 0.048
-
 pH 7.3, 37°C Haemophilus influenzae 3-fluoro-N-[1-(2-methylpropanoyl)-1,2,3,4-tetrahydroquinolin-6-yl]benzene-1-sulfonamide
2.3.1.157 0.066
-
 pH 7.3, 37°C Haemophilus influenzae [4-fluoro-3-[(E)-([5-[hydroxy(oxo)azaniumyl]furan-2-yl]methylidene)amino]phenyl](hydroxy)oxoammonium
2.3.1.157 0.079
-
 pH 7.3, 37°C Haemophilus influenzae 5,7-dichloro-2-hydrazinylquinolin-8-ol
2.3.1.157 0.1
-
 pH 7.3, 37°C Haemophilus influenzae 3-hydrazinylquinoline-2-thiol
2.3.1.157 0.1
-
 pH 7.3, 37°C Haemophilus influenzae (5-[(E)-[(2,5-dimethylphenyl)imino]methyl]furan-2-yl)(hydroxy)oxoammonium

General Information

EC Number General Information Comment Organism
2.3.1.157 metabolism the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Haemophilus influenzae
2.3.1.157 metabolism the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli
2.7.7.23 metabolism the enzyme is involved in the cell wall biosynthesis of Gram-negative organisms Escherichia coli