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Literature summary extracted from

  • Acera, F.; Carmona, M.I.; Castillo, F.; Quesada, A.; Blasco, R.
    A cyanide-induced 3-cyanoalanine nitrilase in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes strain CECT 5344 (2017), Appl. Environ. Microbiol., 83, e00089-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.4 expression in Escherichia coli enables the recombinant strain to fully assimilate 3-cyanoalanine Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.4 Pseudomonas oleovorans W6R265
-
-
3.5.5.4 Pseudomonas oleovorans CECT 5344 W6R265
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.4
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.4 3-cyano-L-alanine + 2 H2O
-
 Pseudomonas oleovorans L-aspartate + NH3
-
 ?
3.5.5.4 3-cyano-L-alanine + 2 H2O
-
 Pseudomonas oleovorans CECT 5344 L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.4 3-cyanoalanine nitrilase
-
 Pseudomonas oleovorans
3.5.5.4 Nit4
-
 Pseudomonas oleovorans

Expression

EC Number Organism Comment Expression
3.5.5.4 Pseudomonas oleovorans cyanide as a sole nitrogen source induces activity up

General Information

EC Number General Information Comment Organism
3.5.5.4 malfunction a nit4 knockout mutant of Pseudomonas pseudoalcaligenes CECT 5344 becomes severely impaired in its ability to grow with 3-cyanoalanine and cyanide as nitrogen sources Pseudomonas oleovorans
3.5.5.4 metabolism the gene cluster including cio1 and nit4 could be involved both in cyanide resistance and in its assimilation by Pseudomonas pseudoalcaligenes CECT 5344 Pseudomonas oleovorans