Literature summary extracted from

Jin, Z.; Di Rienzi, S.C.; Janzon, A.; Werner, J.J.; Angenent, L.T.; Dangl, J.L.; Fowler, D.M.; Ley, R.E.
Novel rhizosphere soil alleles for the enzyme 1-aminocyclopropane-1-carboxylate deaminase queried for function with an in vivo competition assay (2016), Appl. Environ. Microbiol., 82, 1050-1059 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.99.7	identification of a specific ACC deaminase domain region (ACCD-DR) that, when PCR amplified from the soil, produces a variant pool that can be swapped into functional plasmids carrying ACC deaminase-encoding genes. The ACC deaminase domain regions are recombinantly expressed in Escherichia coli. Functional clones of ACC deaminase are selected for in a competition assay based on their capacity to provide nitrogen to Escherichia coli in vitro, DNA and amino acid sequence determination and analysis	uncultured bacterium

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.99.7	uncultured bacterium	-	isolated from rhizosphere soil samples from four maize inbred lines, i.e. Oh43, MS71, M37W, and NC358	-

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.99.7	ACC deaminase	-	uncultured bacterium
3.5.99.7	ACCD	-	uncultured bacterium

General Information

EC Number	General Information	Comment	Organism
3.5.99.7	evolution	library screening and genotyping of rhizosphere soli isolates, sequence comparisons and phylogenetic analysis, overview	uncultured bacterium
3.5.99.7	additional information	identification of a specific ACC deaminase domain region (ACCD-DR) that, when PCR amplified from the soil, produces a variant pool that can be swapped into functional plasmids carrying ACC deaminase-encoding genes. Functional clones of ACC deaminase are selected for in a competition assay based on their capacity to provide nitrogen to Escherichia coli in vitro. Structure-function analysis	uncultured bacterium

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Release 2023.1 (January 2023)