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Literature summary extracted from

  • Abi, A.; Wang, A.; Joerdening, H.J.
    Continuous laminaribiose production using an immobilized bienzymatic system in a packed bed reactor (2018), Appl. Biochem. Biotechnol., 186, 861-876 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.31 D-glucose competitive inhibition Euglena gracilis
2.4.1.31 laminaribiose
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.31 0.001
-
 D-glucose at pH 6.0 and 35°C Euglena gracilis
2.4.1.31 0.00414
-
 laminaribiose at pH 6.0 and 35°C Euglena gracilis
2.4.1.31 0.44
-
 alpha-D-glucose 1-phosphate at pH 6.0 and 35°C Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 Euglena gracilis
-
-
-
2.4.1.31 Euglena gracilis Z
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.31 partially purified using ammonium sulfate precipitation Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 Euglena gracilis laminaribiose + phosphate
-
 ?
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 Euglena gracilis Z laminaribiose + phosphate
-
 ?
2.4.1.31 laminaribiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis laminaritriose + phosphate
-
 ?
2.4.1.31 laminaribiose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis Z laminaritriose + phosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.31 16.65
-
 D-glucose at pH 6.0 and 35°C Euglena gracilis
2.4.1.31 6680
-
 laminaribiose at pH 6.0 and 35°C Euglena gracilis