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Literature summary extracted from

  • Mojica, S.A.; Salin, O.; Bastidas, R.J.; Sunduru, N.; Hedenstroem, M.; Andersson, C.D.; Nunez-Otero, C.; Engstroem, P.; Valdivia, R.H.; Elofsson, M.; Gylfe, A.
    N-acylated derivatives of sulfamethoxazole block chlamydia fatty acid synthesis and interact with FabF (2017), Antimicrob. Agents Chemother., 61, e00716-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.179 expressed in Escherichia coli cells lacking FabF Chlamydia trachomatis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.179 E122K the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides Chlamydia trachomatis
2.3.1.179 G200S the mutant is resistant towards inhibition by acylated sulfonamides Chlamydia trachomatis

General Stability

EC Number General Stability Organism
2.3.1.179 acylated sulfonamides ME0640 (0.5 mM), ME0518 (1 mM) and ME0619 (4 mM) protect the recombinant enzyme from proteolysis Chlamydia trachomatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.179 (2E)-3-(3-chlorophenyl)-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]prop-2-enamide ME0619 Chlamydia trachomatis
2.3.1.179 3,6-dichloro-N-[4-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl]-1-benzothiophene-2-carboxamide ME0640 Chlamydia trachomatis
2.3.1.179 3,6-dichloro-N-[5-[(5-methyl-1,2-oxazol-3-yl)sulfamoyl]pyridin-2-yl]-1-benzothiophene-2-carboxamide ME0518 Chlamydia trachomatis
2.3.1.179 cerulenin
-
 Chlamydia trachomatis
2.3.1.179 additional information not inhibited by triclosan Chlamydia trachomatis
2.3.1.179 platensimycin
-
 Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Chlamydia trachomatis
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Chlamydia trachomatis 434/Bu
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.179 Chlamydia trachomatis A0A0H3MGB3
-
-
2.3.1.179 Chlamydia trachomatis 434/Bu A0A0H3MGB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.179 Ni-NTA agarose column chromatography and HiLoad GF200 gel filtration Chlamydia trachomatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Chlamydia trachomatis a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Chlamydia trachomatis 434/Bu a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.179 3-oxoacyl-(acylcarrier protein) synthase II
-
 Chlamydia trachomatis
2.3.1.179 FabF
-
 Chlamydia trachomatis