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Literature summary extracted from

  • Marapakala, K.; Packianathan, C.; Ajees, A.A.; Dheeman, D.S.; Sankaran, B.; Kandavelu, P.; Rosen, B.P.
    A disulfide-bond cascade mechanism for arsenic(III) S-adenosylmethionine methyltransferase (2015), Acta Crystallogr. Sect. D, 71, 505-515 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expression of a mutant lacking 31 residues from the N-terminus and 28 residues from the C-terminus Cyanidioschyzon sp. 5508

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.137 structure with the bound aromatic arsenicals phenylarsenite at 1.80 A resolution and reduced roxarsone at 2.25 A resolution. Compounds are bound to conserved residues C174 and C224. A loop containing Cys44 and Cys72 shifts by nearly 6.5 A in the arsenic(III)-bound structures compared with the SAM-bound structure Cyanidioschyzon sp. 5508

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.137 C44A mutant is unable to methylate arsenic(III) but retains the ability to methylate methylarsenate Cyanidioschyzon sp. 5508
2.1.1.137 C44A/C72A mutant is unable to methylate arsenic(III) but retains the ability to methylate methylarsenate Cyanidioschyzon sp. 5508

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Cyanidioschyzon sp. 5508 C0JV69
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite
-
 Cyanidioschyzon sp. 5508 S-adenosyl-L-homocysteine + methylarsenate(III)
-
 ?
2.1.1.137 S-adenosyl-L-methionine + phenylarsenite
-
 Cyanidioschyzon sp. 5508 S-adenosyl-L-homocysteine + phenylmethylarsenate(III)
-
 ?