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Literature summary extracted from

  • Zandi, T.A.; Marshburn, R.L.; Stateler, P.K.; Brammer Basta, L.A.
    Phylogenetic and biochemical analyses of mycobacterial L,D-transpeptidases reveal a distinct enzyme class that is preferentially acylated by meropenem (2019), ACS Infect. Dis., 5, 2047-2054 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.B14 homology modeling and docking of faropenem and meropenem. In the LdtF-meropenem model, both hydrogens of the protonated secondary pyrrolidine nitrogen form a bidentate hydrogen bond with Asp353 Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.B14 D353A meropenem preferentially acylates the mutant in a competition experiment Mycolicibacterium smegmatis
2.3.2.B14 D353N meropenem preferentially acylates the mutant in a competition experiment Mycolicibacterium smegmatis
2.3.2.B14 D353W meropenem preferentially acylates the mutant in a competition experiment. Substitution nearly abolishes acylation by biapenem but does not affect acylation by faropenem, doripenem, or meropenem Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.B14 biapenem formation of an acyl adduct Mycolicibacterium smegmatis
2.3.2.B14 doripenem formation of an acyl adduct Mycolicibacterium smegmatis
2.3.2.B14 faropenem formation of an acyl adduct. In competition experiments, both faropenem and meropenem initially acylate LdtF, then faropenem is hydrolyzed off the enzyme at a faster rate than meropenem Mycolicibacterium smegmatis
2.3.2.B14 meropenem complete formation of an acyl adduct. In competition experiments, both faropenem and meropenem initially acylate LdtF, then faropenem is hydrolyzed off the enzyme at a faster rate than meropenem Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.B14 Mycolicibacterium smegmatis
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-
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Synonyms

EC Number Synonyms Comment Organism
2.3.2.B14 LdtF
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 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.3.2.B14 physiological function examination of 323 mycobacterial Ldts and determination into six clades. Mycobacterium smegmatis LdtF belongs to class 6, having structurally divergent catalytic domains containing a 10-residue insertion near the active site and being preferentially acylated by meropenem Mycolicibacterium smegmatis