EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.85 | expression in Escherichia coli | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.85 | additional information | expression of a didomain subconstruct KS-MAT (2?853), bearing a beto-ketoacyl synthase KS knockout C161G and malonyl/acetyltransferase MAT | Mus musculus |
2.3.1.85 | R606A | active site mutant of didomain construct. The specificity constant for malonyl-CoA is 40fold smaller than the wild-type constant | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.85 | 0.00062 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00073 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00086 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00096 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00128 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00136 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00163 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00164 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00172 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.00206 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.0021 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.003 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.0048 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 0.018 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.85 | Mus musculus | P19096 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.85 | 3-hydroxybutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-hydroxybutanoyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | 3-methylbutanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + 3-methylbutanoyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | acetoacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetoacetyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | acetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + acetyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | butanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + butanoyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | crotonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + crotonyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | malonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a malonyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | methylmalonyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + a methylmalonyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | octanoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + octanoyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | palmitoyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + palmitoyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | phenylacetyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + phenylacetyl-[acyl-carrier protein] | - |
? | |
2.3.1.85 | succinyl-CoA + an [acyl-carrier protein] | - |
Mus musculus | CoA + succinyl-[acyl-carrier protein] | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.85 | 2.5 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 4 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 4.1 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 5 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6.1 | - |
3-hydroxybutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 7.2 | - |
3-methylbutanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 7.3 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 10.8 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 13 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 13.4 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 13.7 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 15.6 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.85 | 300 | - |
malonyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 500 | - |
palmitoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 4000 | - |
acetyl-CoA | didomain construct bearing mutation R606A, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 4400 | - |
crotonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 5600 | - |
octanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6200 | - |
phenylacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6400 | - |
succinyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6500 | - |
methylmalonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 6600 | - |
butanoyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 8400 | - |
acetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 8500 | - |
acetoacetyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus | |
2.3.1.85 | 12200 | - |
malonyl-CoA | didomain construct, pH not specified in the publication, 25°C | Mus musculus |