EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.180 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces pactum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.180 | Mg2+ | 10 mM used in assay conditions | Streptomyces pactum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | acetyl-CoA + a malonyl-[acyl-carrier protein] | Streptomyces pactum | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.180 | Streptomyces pactum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.180 | Ni-NTA column chromatography | Streptomyces pactum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.180 | acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Streptomyces pactum | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) (2E)-2,4-dimethylpent-2-enethioate | 44% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl (3E)-3,5-dimethylhex-3-enoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-hydroxy-6-methylbenzene-1-carbothioate | 100% conversion | Streptomyces pactum | 7-deoxy-7-demethylpactamycin + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-methylbutanethioate | 20% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 2-methylbutanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 2-methylpropanethioate | 83% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 2-methylpropanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 3-aminobenzene-1-carbothioate | 17% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 3-aminobenzoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 3-methylbutanethioate | 61% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 3-methylbutanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) 4-chlorobutanethioate | 100% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl 4-chlorobutanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) benzenecarbothioate | 43% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl benzoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) butanethioate | 91% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl butanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) cycloheptanecarbothioate | 87% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl cycloheptanecarboxylate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) cyclohexanecarbothioate | 57% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl cyclohexanecarboxylate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) ethanethioate | 71% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl acetate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) phenylethanethioate | 95% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl phenylacetate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-(6-methylsalicylyl)-7-deoxy-7-demethylpactamycin + S-(2-acetamidoethyl) propanethioate | 87% conversion | Streptomyces pactum | [(1S,2R,3S,4S,5S)-5-(3-acetylanilino)-4-amino-3-[(dimethylcarbamoyl)amino]-1,2-dihydroxy-2,3-dimethylcyclopentyl]methyl propanoate + N-(2-sulfanylethyl)acetamide | - |
? | |
2.3.1.180 | de-6-methylsalicylyl-7-deoxypactamycin + 6-methylsalicylyl N-acetylcysteamine thioester | - |
Streptomyces pactum | ? | - |
? | |
2.3.1.180 | de-6-methylsalicylyl-pactamycate + 6-methylsalicylyl N-acetylcysteamine thioester | - |
Streptomyces pactum | ? | - |
? | |
2.3.1.180 | [PtmQ]-S-methyl 2-methylbenzene-1-carbothioate + de-6-methylsalicylyl-pactamycin | - |
Streptomyces pactum | pactamycin + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.180 | ? | x * 39000, His6-tagged enzyme, SDS-PAGE | Streptomyces pactum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.180 | beta-ketoacyl-ACP synthase III-like protein | - |
Streptomyces pactum |
2.3.1.180 | beta-ketoacyl-acyl carrier protein synthase III | - |
Streptomyces pactum |
2.3.1.180 | KAS III-like protein | - |
Streptomyces pactum |
2.3.1.180 | PtmR | - |
Streptomyces pactum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.180 | metabolism | the enzyme is involved in pactamycin biosynthesis | Streptomyces pactum |