Any feedback?
Literature summary extracted from
- Molecular interplay between the dimer interface and the substrate-binding site of human peptidylarginine deiminase 4 (2017), Sci. Rep., 7, 42662 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.3.15 | expression in Escherichia coli | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.3.15 | C434A | small decrase in kcat/Km value | Homo sapiens |
| 3.5.3.15 | D465A | residue involved in dimerization, about 30% of wild-type catalytic effciency | Homo sapiens |
| 3.5.3.15 | F285AV284A | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | F541A | residue at dimer interface, about 50% decrease in kcat/Km value | Homo sapiens |
| 3.5.3.15 | F576A | residue at dimer interface, about 50% decrease in kcat/Km value | Homo sapiens |
| 3.5.3.15 | L279A | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | L279D | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | L279I | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | L6A | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | L6D | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | L6I | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | R372A | almost complete loss of activity | Homo sapiens |
| 3.5.3.15 | R372K | complete loss of activity | Homo sapiens |
| 3.5.3.15 | R372Q | almost complete loss of activity | Homo sapiens |
| 3.5.3.15 | R441A | residue involved in dimerization, about 6% of wild-type catalytic effciency | Homo sapiens |
| 3.5.3.15 | R639A | about 30% decrease in kcat/Km value | Homo sapiens |
| 3.5.3.15 | V283A | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V283D | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V283I | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V283T | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V284A | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V284D | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V284I | residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity | Homo sapiens |
| 3.5.3.15 | V469A | residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity | Homo sapiens |
| 3.5.3.15 | V469L | residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity | Homo sapiens |
| 3.5.3.15 | V469T | residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity | Homo sapiens |
| 3.5.3.15 | W347A | residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity | Homo sapiens |
| 3.5.3.15 | W347F | residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity | Homo sapiens |
| 3.5.3.15 | W548A | residue at dimer interface, complete loss of activity | Homo sapiens |
| 3.5.3.15 | W548F | residue at dimer interface, about 50% decrease in kcat/Km value | Homo sapiens |
| 3.5.3.15 | W548K | residue at dimer interface, about 50% decrease in kcat/Km value | Homo sapiens |
| 3.5.3.15 | Y435A | residue involved in dimerization, about 4% of wild-type catalytic effciency | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.15 | 0.1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469T, pH 7.5, 25°C | Homo sapiens |  |
| 3.5.3.15 | 0.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548K, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant C434A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant D465A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F576A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | wild-type, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.6 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F541A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R639A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F285A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283T, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469L, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548F, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R441A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 2.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant Y435A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 5.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469A, pH 7.5, 25°C | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.3.15 | Homo sapiens | Q9UM07 | isoform Pad4 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.3.15 | N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Homo sapiens | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.3.15 | More | the dimer interface and the substrate-binding site of PAD4, which consist of the I-loop and the S-loop, respectively, are responsible for substrate binding and dimer stabilization | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.3.15 | PAD4 | - |
Homo sapiens |
| 3.5.3.15 | PADI4 | - |
Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.3.15 | 43 | - |
melting temperature, mutants L6D, L6I | Homo sapiens |
| 3.5.3.15 | 46 | - |
melting temperature, mutant D465A | Homo sapiens |
| 3.5.3.15 | 51 | - |
melting temperature, wild-type | Homo sapiens |
| 3.5.3.15 | 55 | - |
melting temperature, mutant V283T | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.15 | 0.04 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469T, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.07 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469L, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.12 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.6 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548K, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 2.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R441A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 2.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant Y435A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 2.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant D465A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.6 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 4.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R639A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 5.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 5.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283T, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 5.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 6.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548F, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 6.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F285A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 7.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 7.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F541A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 7.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant C434A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 9.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 9.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F576A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 10.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 10.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 11.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 11.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | wild-type, pH 7.5, 25°C | Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.3.15 | 0.02 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.05 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R372Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469L, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.15 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V469T, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 0.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant Y435A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R441A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 1.9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548K, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 3.7 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 4.6 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant W548F, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 6.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283T, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 7.1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R639A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 8.6 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F285A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 8.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant D465A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 9 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6D, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 9.3 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374Q, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 11 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F541A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 11.1 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant R374A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 11.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 12.2 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 13.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 19 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V283I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 20 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant C434A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 21.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L6I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 21.8 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant V284I, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 23.4 | - |
N-alpha-benzoyl-L-arginine ethyl ester | wild-type, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 24.5 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant F576A, pH 7.5, 25°C | Homo sapiens | |
| 3.5.3.15 | 28 | - |
N-alpha-benzoyl-L-arginine ethyl ester | mutant L279I, pH 7.5, 25°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
