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Literature summary extracted from

  • Sanchez-Quitian, Z.; Rodrigues-Junior, V.; Rehm, J.; Eichler, P.; Barbosa Trivella, D.; Bizarro, C.; Basso, L.; Santos, D.
    Functional and structural evidence for the catalytic role played by glutamate-47 residue in the mode of action of Mycobacterium tuberculosis cytidine deaminase (2015), RSC Adv., 5, 830-840 .
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.5 structures of apomutants E47D and E47Q Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.5 E47A mutant protein is insoluble Mycobacterium tuberculosis
3.5.4.5 E47D 37fold decrease in kcat value. No kcat and kcat/KM dependence on pH values ranging from 4.0 to 11 is observed Mycobacterium tuberculosis
3.5.4.5 E47H mutant protein is insoluble Mycobacterium tuberculosis
3.5.4.5 E47L complete loss of activity Mycobacterium tuberculosis
3.5.4.5 E47Q 19fold decrease in kcat value Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 1.004
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 1.461
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 1.612
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.5 Zn2+ presence of 1 mol Zn2+ per mol of subunit Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 55000
-
 PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Mycobacterium tuberculosis P9WPH3
-
-
3.5.4.5 Mycobacterium tuberculosis H37Rv P9WPH3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5 recombinant protein Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 3.46
-
 wild-type, substrate 2'-deoxycytidine, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 4.75
-
 wild-type, substrate cytidine, pH 7.5, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 2'-deoxycytidine + H2O
-
 Mycobacterium tuberculosis 2'-deoxyuridne + NH3
-
 ?
3.5.4.5 2'-deoxycytidine + H2O
-
 Mycobacterium tuberculosis H37Rv 2'-deoxyuridne + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mycobacterium tuberculosis uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mycobacterium tuberculosis H37Rv uridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 tetramer 4 * 13900, SDS-PAGE Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.5 0.13
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 0.26
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 4.85
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.5 0.08
-
 cytidine mutant E47D, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 0.178
-
 cytidine mutant E47Q, pH 7.5, 25°C Mycobacterium tuberculosis
3.5.4.5 4.83
-
 cytidine wild-type, pH 7.5, 25°C Mycobacterium tuberculosis