EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.11 | synthesis | enzyme immobilized on alginate/chitosan hybrid beads can be used on large scale for the synthesis of beta-lactam | Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
3.5.1.11 | enzyme immobilized on alginate/chitosan hybrid beads can be reused more times as compared to enzyme immobilized on alginate beads | Escherichia coli |
3.5.1.11 | enzyme immobilized on alginate/chitosan hybrid beads shows higher operational stability compared to enzyme immobilized on alginate beads | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.11 | 56.19 | - |
penicillin G | soluble enzyme, pH and temperature not specified in the publication | Escherichia coli | |
3.5.1.11 | 64.84 | - |
penicillin G | enzyme immobilized on alginate beads, pH and temperature not specified in the publication | Escherichia coli | |
3.5.1.11 | 87.08 | - |
penicillin G | enzyme immobilized on alginate/chitosan hybrid beads, pH and temperature not specified in the publication | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.11 | Escherichia coli | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.11 | enzyme immobilized on alginate/chitosan hybrid beads shows higher storage stability compared to enzyme immobilized on alginate beads | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.11 | Penicillin G amidase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 40 | - |
soluble enzyme | Escherichia coli |
3.5.1.11 | 50 | - |
enzyme immobilized on alginate/chitosan hybrid beads and enzyme immobilized on alginate beads | Escherichia coli |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 30 | 70 | 30°C: about 85% of maximal activity, 70°C: about 85% of maximal activity, enzyme immobilized on alginate/chitosan hybrid beads | Escherichia coli |
3.5.1.11 | 30 | 70 | 30°C: about 95% of maximal activity, 70°C: about 65% of maximal activity, soluble enzyme | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 70 | - |
thermal stability of the enzyme immobilized on alginate/chitosan hybrid beads is much higher that that of the soluble enzyme | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 10 | - |
stability of the enzyme immobilized on alginate/chitosan hybrid beads is higher that that of the enzyme immobilized an alginate. | Escherichia coli |