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Literature summary extracted from

  • Yan, L.; Jin, T.; Chen, Y.; Zhan, C.; Zhang, L.; Weng, L.; Liu, G.; Cao, M.
    Characterization of a recombinant matrix metalloproteinase-2 from sea cucumber (Stichopus japonicas) and its application to prepare bioactive collagen hydrolysate (2018), Process Biochem., 72, 63-70 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.24 synthesis application of MMP-2 from sea cucumber (Stichopus japonicas) to prepare bioactive collagen hydrolysate Apostichopus japonicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.24 MMP-2 gene cloning from the body wall of sea cucumber, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged MMP-2(111-461) comprising the catalytic domain in Escherichia coli strain BL21(DE3) in inclusion bodies Apostichopus japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.24 Ba2+ can only partially complement Ca2+ for enzyme activity Apostichopus japonicus
3.4.24.24 Ca2+ Ca2+ is required for optimal gelatinolytic activity, binding structure, overview Apostichopus japonicus
3.4.24.24 additional information no activity with Fe2+, Co2+, Cu2+, Mn2+ or Mg2+ Apostichopus japonicus
3.4.24.24 Zn2+ dependent on, binding structure, overview, can only partially complement Ca2+ for enzyme activity Apostichopus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.24 Collagen + H2O Apostichopus japonicus degradation of beta- and gamma-chains, weak activity with alpha-chains ?
-
 ?
3.4.24.24 Gelatin + H2O Apostichopus japonicus
-
 ?
-
 ?
3.4.24.24 additional information Apostichopus japonicus rMMP-2 degrades collagen effectively and the hydrolysate reveals scavenging activities on both 2, 2'-diphenyl-1-picrylhydrazyl free radical and hydrogen peroxide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.24 Apostichopus japonicus A0A2P9J4V0 i.e. Stichopus japonicas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.24 recombinant soluble, refolded His-tagged MMP-2(111-461) from Escherichia coli by nickel affinity chromatography (eluted with 50 mM Tris-HCl, pH 8.0, containing 300 mM NaCl, 4 M urea, and 200 mM imidazole) and dialysis Apostichopus japonicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.24 refolding of recombinant His-tagged MMP-2(111-461) from Escherichia coli strain BL21(DE3) dissolved with 4 M urea from inclusion bodies Apostichopus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.24 Collagen + H2O degradation of beta- and gamma-chains, weak activity with alpha-chains Apostichopus japonicus ?
-
 ?
3.4.24.24 Gelatin + H2O
-
 Apostichopus japonicus ?
-
 ?
3.4.24.24 additional information rMMP-2 degrades collagen effectively and the hydrolysate reveals scavenging activities on both 2, 2'-diphenyl-1-picrylhydrazyl free radical and hydrogen peroxide Apostichopus japonicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.24 ? x * 40000, recombinant MMP-2(111-461), SDS-PAGE, x * 70890, sequence calculation Apostichopus japonicus
3.4.24.24 More the deduced amino acid sequence of MMP-2 contains a signal peptide, a propeptide domain, a catalytic domain with three repeats of fibronectin-type II region and a C-terminal hemopexin-like domain. Three-dimensional and secondary enzyme structure analysis Apostichopus japonicus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.24 matrix metalloproteinase-2
-
 Apostichopus japonicus
3.4.24.24 MMP-2
-
 Apostichopus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.24 40
-
 recombinant enzyme Apostichopus japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.24 56.8
-
 denaturation temperature of recombinant MMP-2 Apostichopus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.24 8.5
-
 recombinant enzyme Apostichopus japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.24 7 10 activity range, recombinant enzyme, no activity below or above Apostichopus japonicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.24 Apostichopus japonicus sequence calculation
-
 5.05