BRENDA - Enzyme Database

Preparation of minor ginsenosides C-Mx and C-K from notoginseng leaf ginsenosides by a special ginsenosidase type-I

Liu, C.; Zuo, K.; Yu, H.; Sun, C.; Zhang, T.; Xu, L.; Jin, Y.; Im, W.; Jin, F.; Process Biochem. 50, 2158-2167 (2015)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.193
additional information
-
additional information
Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc
Aspergillus niger
3.2.1.193
5.46
-
ginsenoside Rc
pH 5.0, 45°C
Aspergillus niger
3.2.1.193
15.1
-
ginsenoside Rb3
pH 5.0, 45°C, formation of compound Mx-1
Aspergillus niger
3.2.1.193
16.6
-
ginsenoside Rb1
pH 5.0, 45°C
Aspergillus niger
3.2.1.193
16.6
-
ginsenoside Rb3
pH 5.0, 45°C, formation of ginsenoside Rd
Aspergillus niger
3.2.1.193
20.4
-
ginsenoside Rb2
pH 5.0, 45°C
Aspergillus niger
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.193
compound Mc + H2O
Aspergillus niger
-
compound K + L-arabinofuranose
-
-
?
3.2.1.193
compound Mc1 + H2O
Aspergillus niger
-
compound Mc + D-glucose
-
-
?
3.2.1.193
compound Mx + H2O
Aspergillus niger
-
compound K + D-xylose
-
-
?
3.2.1.193
compound Mx-1 + H2O
Aspergillus niger
-
compound Mx + D-glucose
-
-
?
3.2.1.193
compound O + H2O
Aspergillus niger
-
compound Y + D-glucose
-
-
?
3.2.1.193
compound Y + H2O
Aspergillus niger
-
compound K + L-arabinopyranose
-
-
?
3.2.1.193
ginsenoside F2 + H2O
Aspergillus niger
-
compound K + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb2 + H2O
Aspergillus niger
-
compound O + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger
-
ginsenoside Rd + D-xylose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger
-
compound Mx1 + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger g.848
-
ginsenoside Rd + D-xylose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger
-
ginsenoside Rd + L-arabinofuranose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger
-
compound Mc1 + D-glucose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger g.848
-
ginsenoside Rd + L-arabinofuranose
-
-
?
3.2.1.193
ginsenoside Rd + H2O
Aspergillus niger
-
ginsenoside F2 + D-glucose
-
-
?
3.2.1.193
additional information
Aspergillus niger
substrates are notoginseng leaf ginsenosides
?
-
-
?
3.2.1.193
additional information
Aspergillus niger g.848
substrates are notoginseng leaf ginsenosides
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.193
Aspergillus niger
-
-
-
3.2.1.193
Aspergillus niger g.848
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.193
native enzyme from mycelium by anion exchange chromatography to homogeneity
Aspergillus niger
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.193
compound Mc + H2O
-
755274
Aspergillus niger
compound K + L-arabinofuranose
-
-
-
?
3.2.1.193
compound Mc1 + H2O
-
755274
Aspergillus niger
compound Mc + D-glucose
-
-
-
?
3.2.1.193
compound Mx + H2O
-
755274
Aspergillus niger
compound K + D-xylose
-
-
-
?
3.2.1.193
compound Mx-1 + H2O
-
755274
Aspergillus niger
compound Mx + D-glucose
-
-
-
?
3.2.1.193
compound O + H2O
-
755274
Aspergillus niger
compound Y + D-glucose
-
-
-
?
3.2.1.193
compound Y + H2O
-
755274
Aspergillus niger
compound K + L-arabinopyranose
-
-
-
?
3.2.1.193
ginsenoside F2 + H2O
-
755274
Aspergillus niger
compound K + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb2 + H2O
-
755274
Aspergillus niger
compound O + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-xylose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-xylose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
compound Mx1 + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger
ginsenoside Rd + L-arabinofuranose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + L-arabinofuranose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger
compound Mc1 + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rd + H2O
-
755274
Aspergillus niger
ginsenoside F2 + D-glucose
-
-
-
?
3.2.1.193
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger
?
-
-
-
?
3.2.1.193
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger
?
-
-
-
?
3.2.1.193
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger g.848
?
-
-
-
?
3.2.1.193
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger g.848
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.193
?
x * 75000, SDS-PAGE
Aspergillus niger
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.193
ginsenosidase type-I
-
Aspergillus niger
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.193
45
-
-
Aspergillus niger
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.193
5
-
-
Aspergillus niger
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.193
additional information
-
additional information
Vmax is 79.6 mM/h for Rb1, 45.6 mM/h for Rb2, 1.62 mM/h for Rb3 to Rd, 10.8 mM/h for Rb3 to C-Mx1, and 6.16 mM/h for Rc
Aspergillus niger
3.2.1.193
5.46
-
ginsenoside Rc
pH 5.0, 45°C
Aspergillus niger
3.2.1.193
15.1
-
ginsenoside Rb3
pH 5.0, 45°C, formation of compound Mx-1
Aspergillus niger
3.2.1.193
16.6
-
ginsenoside Rb1
pH 5.0, 45°C
Aspergillus niger
3.2.1.193
16.6
-
ginsenoside Rb3
pH 5.0, 45°C, formation of ginsenoside Rd
Aspergillus niger
3.2.1.193
20.4
-
ginsenoside Rb2
pH 5.0, 45°C
Aspergillus niger
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.193
compound Mc + H2O
Aspergillus niger
-
compound K + L-arabinofuranose
-
-
?
3.2.1.193
compound Mc1 + H2O
Aspergillus niger
-
compound Mc + D-glucose
-
-
?
3.2.1.193
compound Mx + H2O
Aspergillus niger
-
compound K + D-xylose
-
-
?
3.2.1.193
compound Mx-1 + H2O
Aspergillus niger
-
compound Mx + D-glucose
-
-
?
3.2.1.193
compound O + H2O
Aspergillus niger
-
compound Y + D-glucose
-
-
?
3.2.1.193
compound Y + H2O
Aspergillus niger
-
compound K + L-arabinopyranose
-
-
?
3.2.1.193
ginsenoside F2 + H2O
Aspergillus niger
-
compound K + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb2 + H2O
Aspergillus niger
-
compound O + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger
-
ginsenoside Rd + D-xylose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger
-
compound Mx1 + D-glucose
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
Aspergillus niger g.848
-
ginsenoside Rd + D-xylose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger
-
ginsenoside Rd + L-arabinofuranose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger
-
compound Mc1 + D-glucose
-
-
?
3.2.1.193
ginsenoside Rc + H2O
Aspergillus niger g.848
-
ginsenoside Rd + L-arabinofuranose
-
-
?
3.2.1.193
ginsenoside Rd + H2O
Aspergillus niger
-
ginsenoside F2 + D-glucose
-
-
?
3.2.1.193
additional information
Aspergillus niger
substrates are notoginseng leaf ginsenosides
?
-
-
?
3.2.1.193
additional information
Aspergillus niger g.848
substrates are notoginseng leaf ginsenosides
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.193
native enzyme from mycelium by anion exchange chromatography to homogeneity
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.193
compound Mc + H2O
-
755274
Aspergillus niger
compound K + L-arabinofuranose
-
-
-
?
3.2.1.193
compound Mc1 + H2O
-
755274
Aspergillus niger
compound Mc + D-glucose
-
-
-
?
3.2.1.193
compound Mx + H2O
-
755274
Aspergillus niger
compound K + D-xylose
-
-
-
?
3.2.1.193
compound Mx-1 + H2O
-
755274
Aspergillus niger
compound Mx + D-glucose
-
-
-
?
3.2.1.193
compound O + H2O
-
755274
Aspergillus niger
compound Y + D-glucose
-
-
-
?
3.2.1.193
compound Y + H2O
-
755274
Aspergillus niger
compound K + L-arabinopyranose
-
-
-
?
3.2.1.193
ginsenoside F2 + H2O
-
755274
Aspergillus niger
compound K + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb1 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb2 + H2O
-
755274
Aspergillus niger
compound O + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
ginsenoside Rd + D-xylose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + D-xylose
-
-
-
?
3.2.1.193
ginsenoside Rb3 + H2O
-
755274
Aspergillus niger
compound Mx1 + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger
ginsenoside Rd + L-arabinofuranose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger g.848
ginsenoside Rd + L-arabinofuranose
-
-
-
?
3.2.1.193
ginsenoside Rc + H2O
-
755274
Aspergillus niger
compound Mc1 + D-glucose
-
-
-
?
3.2.1.193
ginsenoside Rd + H2O
-
755274
Aspergillus niger
ginsenoside F2 + D-glucose
-
-
-
?
3.2.1.193
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger
?
-
-
-
?
3.2.1.193
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger
?
-
-
-
?
3.2.1.193
additional information
substrates are notoginseng leaf ginsenosides
755274
Aspergillus niger g.848
?
-
-
-
?
3.2.1.193
additional information
the enzyme first hydrolyzes the C-20 position 20-O-Glc of ginsenoside Rb1, and then C-3 position 3-O-Glc via a single pathway: Rb1 -> Rd -> F2 -> C-K. It first hydrolyzes 3-O-Glc of ginsenoside Rb2 and Rc, and then 20-O-Ara via a single pathway: Rb2 -> C-O -> C-Y -> C-K and Rc -> C-Mc1 -> C-Mc -> C-K. It mainly hydrolyzes 3-O-Glc of Rb3 via pathway: Rb3 -> C-Mx1 -> C-Mx -> C-K, and slowly hydrolyzes 20-O-Xyl of Rb3 via pathway Rb3 -> Rd -> F2 -> C-K. The average mole yields are 48.2% for compound Mx from ginsenoside Rb3 and compound Mx1, and 41.2% for compound K from other ginsenosides. Products are identified by by TLC and HPLC analysis and NMR spectroscopy. The ability of the pure enzyme to hydrolyze 3-O- and 20-O-glucoside proves that it is a ginsenosidase type-I
755274
Aspergillus niger g.848
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.193
?
x * 75000, SDS-PAGE
Aspergillus niger
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.193
45
-
-
Aspergillus niger
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.193
5
-
-
Aspergillus niger