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Literature summary extracted from

  • Shinji, T.; Moe, Y.; Yukihiro, K.; Yoko, Y.; Hitoshi, A.
    Characterization of an organic-solvent-stable elastase from Pseudomonas indica and its potential use in eggshell membrane hydrolysis (2019), Proc. Biochem., 85, 156-163 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.26 sodium sulfite enhances hydrolysis of eggshell-membrane Pseudomonas indica

Application

EC Number Application Comment Organism
3.4.24.26 synthesis the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane. Potential applicability of its peptides as functional food and cosmetic additives Pseudomonas indica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.26 expression in Escherichia coli Pseudomonas indica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 CH2ICOOH 3 mM,88 % inhibition Pseudomonas indica
3.4.24.26 DTT 3 mM, 24% inhibition Pseudomonas indica
3.4.24.26 EDTA 3 mM, 14% inhibition Pseudomonas indica
3.4.24.26 phosphoramidon 3 mM, 13% inhibition Pseudomonas indica
3.4.24.26 PMSF 5 mM, 27% inhibition Pseudomonas indica
3.4.24.26 sodium sulfite 3 mM, 94% inhibition Pseudomonas indica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 extracellular
-
 Pseudomonas indica
-
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.26 2-propanol 25% (v/v), 1 week, stable Pseudomonas indica
3.4.24.26 Acetone 25% (v/v), 1 week, stable Pseudomonas indica
3.4.24.26 DMSO 25% (v/v), 1 week, stable Pseudomonas indica
3.4.24.26 Ethanol 25% (v/v), 1 week, stable Pseudomonas indica
3.4.24.26 Ethyl acetate 25% (v/v), 1 week, stable Pseudomonas indica
3.4.24.26 hexane 25% (v/v), 1 week, stable Pseudomonas indica

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas indica WP_084338031.1
-
-
3.4.24.26 Pseudomonas indica NBRC 103045 WP_084338031.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas indica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.26 culture fluid
-
 Pseudomonas indica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 casein + H2O
-
 Pseudomonas indica ?
-
 ?
3.4.24.26 casein + H2O
-
 Pseudomonas indica NBRC 103045 ?
-
 ?
3.4.24.26 eggshell-membrane + H2O the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane Pseudomonas indica ?
-
 ?
3.4.24.26 eggshell-membrane + H2O the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane Pseudomonas indica NBRC 103045 ?
-
 ?
3.4.24.26 elasti-orcein + H2O
-
 Pseudomonas indica ?
-
 ?
3.4.24.26 elasti-orcein + H2O
-
 Pseudomonas indica NBRC 103045 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.26 LasB
-
 Pseudomonas indica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.26 45
-
 hydrolysis of eggshell-membrane Pseudomonas indica
3.4.24.26 55
-
 casein hydrolysis Pseudomonas indica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.26 30 55 stable Pseudomonas indica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.26 8 9
-
 Pseudomonas indica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.26 4.5 12 stable Pseudomonas indica