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Literature summary extracted from

  • Sabljic, I.; Tomin, M.; Matovina, M.; Sucec, I.; Tomacic Paic, A.; Tomic, A.; Abramic, M.; Tomic, S.
    The first dipeptidyl peptidase III from a thermophile Structural basis for thermal stability and reduced activity (2018), PLoS ONE, 13, e0192488 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.14.4
-
 Caldithrix abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.4 EDTA complete loss of activity Caldithrix abyssi
3.4.14.4 additional information activities in phosphate buffers are singnificantly lower than in Tris-Hcl buffer Caldithrix abyssi
3.4.14.4 o-phenanthroline complete loss of activity Caldithrix abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.4 0.031
-
 Arg-Arg-2-naphtylamide pH 7.0, 25°C Caldithrix abyssi
3.4.14.4 0.035
-
 Arg-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi
3.4.14.4 0.329
-
 Gly-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.14.4 Zinc the zinc ion is essential for the enzyme activity. It is positioned in the lower part of the upper domain where it is pentacoordinated by two histidines Caldithrix abyssi

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.4 Caldithrix abyssi H1XW48
-
-
3.4.14.4 Caldithrix abyssi DSM 13497 H1XW48
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.4 Ala-Ala-2-naphtylamide + H2O 103% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi Ala-Ala + 2-naphthylamine
-
 ?
3.4.14.4 Ala-Ala-2-naphtylamide + H2O 103% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi DSM 13497 Ala-Ala + 2-naphthylamine
-
 ?
3.4.14.4 Ala-Arg-2-naphtylamide + H2O 65% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi Ala-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Ala-Arg-2-naphtylamide + H2O 65% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi DSM 13497 Ala-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Arg-Arg-2-naphtylamide + H2O
-
 Caldithrix abyssi Arg-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Arg-Arg-2-naphtylamide + H2O
-
 Caldithrix abyssi DSM 13497 Arg-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Gly-Arg-2-naphtylamide + H2O 167% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi Gly-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Gly-Arg-2-naphtylamide + H2O 167% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi DSM 13497 Gly-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Phe-Arg-2-naphtylamide + H2O 129% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi Phe-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Phe-Arg-2-naphtylamide + H2O 129% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi DSM 13497 Phe-Arg + 2-naphthylamine
-
 ?
3.4.14.4 Pro-Arg-2-naphtylamide + H2O 143% of the activity with Arg-Arg-2-naphtylamide Caldithrix abyssi Pro-Arg + 2-naphthylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.14.4 Cabys_2252
-
 Caldithrix abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.4 50
-
-
 Caldithrix abyssi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.4 0.2
-
 Arg-Arg-2-naphtylamide pH 7.0, 25°C Caldithrix abyssi
3.4.14.4 2.53
-
 Gly-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi
3.4.14.4 3.07
-
 Arg-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.4 7
-
-
 Caldithrix abyssi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.4 6.63
-
 Arg-Arg-2-naphtylamide pH 7.0, 25°C Caldithrix abyssi
3.4.14.4 7.67
-
 Gly-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi
3.4.14.4 87.3
-
 Arg-Arg-2-naphtylamide pH 7.0, 50°C Caldithrix abyssi