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Literature summary extracted from

  • Han, Y.; Zhu, Q.; Zhang, Z.; Meng, K.; Hou, Y.; Ban, Q.; Suo, J.; Rao, J.
    Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes and diverse roles of isoenzymes during persimmon fruit development and postharvest softening (2015), PLoS ONE, 10, e0123668 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.207 gene XTH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
2.4.1.207 gene XTH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
3.2.1.151 gene XTH3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
3.2.1.151 gene XTH4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki
3.2.1.151 gene XTH5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Diospyros kaki

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.207 cell wall the coding protein of DkXTH1 is targeted to the cell wall by its N-terminal signal peptide Diospyros kaki 5618
-
2.4.1.207 cell wall the coding protein of DkXTH2 is targeted to the cell wall by its N-terminal signal peptide Diospyros kaki 5618
-
2.4.1.207 additional information the enzyme contains a signal peptide Diospyros kaki
-
-
3.2.1.151 cell wall
-
 Diospyros kaki 5618
-
3.2.1.151 additional information the enzyme contains a signal peptide Diospyros kaki
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.207 xyloglucan Diospyros kaki
-
 ?
-
 ?
3.2.1.151 xyloglucan + H2O Diospyros kaki
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Diospyros kaki G5DAC6 cv. Fupingjianshi, i.e. Diospyros chinensis
-
2.4.1.207 Diospyros kaki G5DAC7 cv. Fupingjianshi, i.e. Diospyros chinensis
-
3.2.1.151 Diospyros kaki A0A067XSJ2 cv. Fupingjianshi, i.e. Diospyros chinensis
-
3.2.1.151 Diospyros kaki G5DAC8 cv. Fuping Jianshi, i.e. Diospyros chinensis
-
3.2.1.151 Diospyros kaki G5DAC9 cv. Fupingjianshi, i.e. Diospyros chinensis
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 glycoprotein the enzyme contains a potential N-linked glycosylation (N-X-S/T) site Diospyros kaki
3.2.1.151 glycoprotein the enzyme contains a potential N-linked glycosylation (N-X-S/T) site Diospyros kaki

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis Diospyros kaki
3.2.1.151 native enzyme by ultrafiltration, dialysis, and gel filtration, recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis Diospyros kaki

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 fruit expression analysis of isozymes DkXTH1-5 during Fuping Jianshi fruit development, expression patterns, overview Diospyros kaki
-
3.2.1.151 fruit expression analysis of isozymes DkXTH1-5 during Fuping Jianshi fruit development, expression patterns, overview Diospyros kaki
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 additional information no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH1 Diospyros kaki ?
-
 ?
2.4.1.207 additional information no hydrolytic activity with xyloglucan (EC 3.2.1.151) by isozyme DkXTH2 Diospyros kaki ?
-
 ?
2.4.1.207 tamarind xyloglucan 3% tamarind xyloglucan Diospyros kaki ?
-
 ?
2.4.1.207 xyloglucan
-
 Diospyros kaki ?
-
 ?
3.2.1.151 tamarind xyloglucan + H2O 3% tamarind xyloglucan Diospyros kaki ?
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Diospyros kaki ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.207 ? x * 32550, sequence calculation Diospyros kaki
2.4.1.207 ? x * 34320, sequence calculation Diospyros kaki
3.2.1.151 ? x * 31930, sequence calculation Diospyros kaki
3.2.1.151 ? x * 32340, sequence calculation Diospyros kaki
3.2.1.151 ? x * 33530, sequence calculation Diospyros kaki

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 DKXTH1
-
 Diospyros kaki
2.4.1.207 DKXTH2
-
 Diospyros kaki
2.4.1.207 XET
-
 Diospyros kaki
2.4.1.207 XTH
-
 Diospyros kaki
2.4.1.207 xyloglucan endotransglycosylase
-
 Diospyros kaki
2.4.1.207 xyloglucan endotransglycosylase/hydrolase
-
 Diospyros kaki
3.2.1.151 DkXTH3
-
 Diospyros kaki
3.2.1.151 DkXTH4
-
 Diospyros kaki
3.2.1.151 DkXTH5
-
 Diospyros kaki
3.2.1.151 More cf. EC 2.4.1.207 Diospyros kaki
3.2.1.151 XEH
-
 Diospyros kaki
3.2.1.151 XTH
-
 Diospyros kaki
3.2.1.151 xyloglucan endohydrolase
-
 Diospyros kaki
3.2.1.151 xyloglucan endotransglycosylase/hydrolase
-
 Diospyros kaki

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 37
-
 assay at Diospyros kaki
3.2.1.151 37
-
 assay at Diospyros kaki

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 4.5 6.5
-
 Diospyros kaki
2.4.1.207 5 5.5
-
 Diospyros kaki
3.2.1.151 5.5
-
 assay at Diospyros kaki

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.207 4 6 isozyme DkXTH1 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs when the pH ranges from pH 4 to pH 5, which is typical for XET isoenzymes Diospyros kaki
2.4.1.207 4 7 isozyme DkXTH2 exhibits a bell-shaped pH profile, and a rapid loss of activity occurs below pH 4, which is typical for XET isoenzymes Diospyros kaki

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.207 Diospyros kaki sequence calculation
-
 8.66
2.4.1.207 Diospyros kaki sequence calculation
-
 9.28
3.2.1.151 Diospyros kaki sequence calculation
-
 5.36
3.2.1.151 Diospyros kaki sequence calculation
-
 6.45
3.2.1.151 Diospyros kaki sequence calculation
-
 8.94

General Information

EC Number General Information Comment Organism
2.4.1.207 evolution the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH Diospyros kaki
2.4.1.207 additional information three-dimensional structure modelling of DkXTH1 based on the template of the crystal structure of PttXET16A (PDB ID 1un1) Diospyros kaki
2.4.1.207 additional information three-dimensional structure modelling of DkXTH2 based on the template of the crystal structure of PttXET16A (PDB ID 1un1) Diospyros kaki
2.4.1.207 physiological function several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity Diospyros kaki
3.2.1.151 evolution the XTH isozymes contain the conserved DEIDFEFLG motif identified as the catalytic domain of XTH Diospyros kaki
3.2.1.151 physiological function several XTH isozymes function in persimmon (Diospyros kaki) fruit development and postharvest softening. Isozymes DkXTH1, DkXTH4, and DkXTH5 expressions peak in immature expanding fruit, and their higher expression is observed along with higher fruit firmness in cold-treated fruit or firmer cultivar fruit during storage. The opposite gene expression patterns are observed in DkXTH2 and DkXTH3, which reach maxima concomitance with pronounced fruit softening. Meanwhile, the xyloglucan endotransglycosylase (XET, EC 2.4.1.207) enzymes play important roles in both the rapid growth and ripening of persimmon fruit. Furthermore, the recombined DkXTH1 and DkXTH2 proteins show significant XET activity without any detected XEH (EC 3.2.1.151) activity Diospyros kaki