Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shekar, S.; Tumaney, A.W.; Rao, T.J.; Rajasekharan, R.
    Isolation of lysophosphatidic acid phosphatase from developing peanut cotyledons (2002), Plant Physiol., 128, 988-996 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.106 octylglucoside 10 mM, 2fold incraese in activity Arachis hypogaea
3.1.3.106 Triton X-100 10 mM, 2fold incraese in activity Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.106 CHAPS 2 mM, 50% decrease in activity Arachis hypogaea
3.1.3.106 deoxycholate 2 mM, 50% decrease in activity Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.106 0.0286
-
 oleoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea
3.1.3.106 0.0393
-
 stearoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea
3.1.3.106 0.0479
-
 palmitoyl-sn-glycerol 3-phosphate pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.106 soluble
-
 Arachis hypogaea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.106 Ca2+ 8 mM, 40-50% inhibition Arachis hypogaea
3.1.3.106 Mn2+ 8 mM, 40-50% inhibition Arachis hypogaea
3.1.3.106 Zn2+ 8 mM, 40-50% inhibition Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.106 39000
-
 gel filtration Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.106 oleoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 oleoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 palmitoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 palmitoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 stearoyl-sn-glycerol 3-phosphate + H2O Arachis hypogaea
-
 stearoyl-sn-glycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.106 Arachis hypogaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.106 partial Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.106 cotyledon
-
 Arachis hypogaea
-
3.1.3.106 leaf
-
 Arachis hypogaea
-
3.1.3.106 seed immature seed Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.106 858
-
 substrate oleoyl-sn-glycerol 3-phosphate, pH 7.0, 30°C, presence of Triton X-100 Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.106 additional information the enzyme is specific to lysophosphatidic acid and does not utilize any other substrate such as glycerol-3-phosphate, phosphatidic acid, or p-nitrophenylphosphate Arachis hypogaea ?
-
 ?
3.1.3.106 oleoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea oleoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 palmitoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea palmitoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 stearoyl-sn-glycerol 3-phosphate + H2O
-
 Arachis hypogaea stearoyl-sn-glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.106 monomer 1 * 39000, SDS-PAGE Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
3.1.3.106 LPA phosphatase
-
 Arachis hypogaea
3.1.3.106 lysophosphatidic acid phosphatase
-
 Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.106 7
-
-
 Arachis hypogaea