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Literature summary extracted from

  • Akoury, E.; Ma, G.; Demolin, S.; Broenner, C.; Zocco, M.; Cirilo, A.; Ivic, N.; Halic, M.
    Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity (2019), Nucleic Acids Res., 47, 6726-6736 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.355 expressed in Escherichia coli BL21(DE3) cells Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.355 RNA the presence of RNA strongly inhibits enzyme activity in vitro Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.355 Mg2+ 1 mM used in assay conditions Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.355 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 Schizosaccharomyces pombe overall reaction 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9 Schizosaccharomyces pombe
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.355 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.355 Ni-NTA column chromatography and Superdex S200 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.355 3 S-adenosyl-L-methionine + a [histone H3]-L-lysine9 overall reaction Schizosaccharomyces pombe 3 S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine9
-
 ?
2.1.1.355 S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine9
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine9
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.355 ? x * 55920, calculated from amino acid sequence Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
2.1.1.355 Clr4
-
 Schizosaccharomyces pombe
2.1.1.355 H3K9 methyltransferase
-
 Schizosaccharomyces pombe

General Information

EC Number General Information Comment Organism
2.1.1.355 physiological function the enzyme contributes to heterochromatin formation Schizosaccharomyces pombe