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Literature summary extracted from

  • Gauss, R.; Kanehara, K.; Carvalho, P.; Ng, D.; Aebi, M.
    A complex of Pdi1p and the mannosidase Htm1p initiates clearance of unfolded glycoproteins from the endoplasmic reticulum (2011), Mol. Cell., 42, 782-793 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.210 expressed in Sf9 insect cells Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.210 endoplasmic reticulum
-
 Saccharomyces cerevisiae 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.210 Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + H2O Saccharomyces cerevisiae
-
 Man7GlcNAc2-[protein] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + H2O Saccharomyces cerevisiae W303a
-
 Man7GlcNAc2-[protein] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.210 Saccharomyces cerevisiae
-
-
-
3.2.1.210 Saccharomyces cerevisiae W303a
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.210 GSH-Sepharose bead chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.210 Man8GlcNAc2-[carboxypeptidase Y] + H2O
-
 Saccharomyces cerevisiae Man7GlcNAc2-[carboxypeptidase Y] + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[carboxypeptidase Y] + H2O
-
 Saccharomyces cerevisiae W303a Man7GlcNAc2-[carboxypeptidase Y] + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[glucanosyl transferase Gas1p] (isomer 8A1,2,3B1,3) + H2O
-
 Saccharomyces cerevisiae Man7GlcNAc2-[glucanosyl transferase Gas1p] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[glucanosyl transferase Gas1p] (isomer 8A1,2,3B1,3) + H2O
-
 Saccharomyces cerevisiae W303a Man7GlcNAc2-[glucanosyl transferase Gas1p] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + H2O
-
 Saccharomyces cerevisiae Man7GlcNAc2-[protein] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?
3.2.1.210 Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + H2O
-
 Saccharomyces cerevisiae W303a Man7GlcNAc2-[protein] (isomer 7A1,2,3B3) + D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.210 ? x * 150000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.210 Htm1p
-
 Saccharomyces cerevisiae
3.2.1.210 Mnl1p
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
3.2.1.210 metabolism a complex of the yeast protein disulfide isomerase Pdi1p and the enzyme Htm1p processes Man8GlcNAc2 carbohydrates bound to unfolded proteins, yielding Man7GlcNAc2. This glycan serves as a signal for HRD ligase-mediated glycoprotein disposal Saccharomyces cerevisiae