EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.24 | gene bsh, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strains BLR(DE3) and XL-1 Blue | Lactiplantibacillus plantarum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.24 | N79V | site-directed mutagenesis, the V79 mutation results in a stable enzyme, but reduces the catalytic activity and alters the substrate specificity of the enzyme | Lactiplantibacillus plantarum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | glycochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
chenodeoxycholate + glycine | - |
? | |
3.5.1.24 | glycochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum B14 | - |
chenodeoxycholate + glycine | - |
? | |
3.5.1.24 | glycocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + glycine | - |
? | |
3.5.1.24 | glycocholic acid + H2O | Lactiplantibacillus plantarum B14 | - |
cholate + glycine | - |
? | |
3.5.1.24 | glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
deoxycholate + glycine | - |
? | |
3.5.1.24 | glycodeoxycholic acid + H2O | Lactiplantibacillus plantarum B14 | - |
deoxycholate + glycine | - |
? | |
3.5.1.24 | taurochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
chenodeoxycholate + taurine | - |
? | |
3.5.1.24 | taurochenodeoxycholic acid + H2O | Lactiplantibacillus plantarum B14 | - |
chenodeoxycholate + taurine | - |
? | |
3.5.1.24 | taurocholic acid + H2O | Lactiplantibacillus plantarum | - |
cholate + taurine | - |
? | |
3.5.1.24 | taurodeoxycholic acid + H2O | Lactiplantibacillus plantarum | - |
deoxycholate + taurine | - |
? | |
3.5.1.24 | taurodeoxycholic acid + H2O | Lactiplantibacillus plantarum B14 | - |
deoxycholate + taurine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.24 | Lactiplantibacillus plantarum | - |
- |
- |
3.5.1.24 | Lactiplantibacillus plantarum B14 | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.24 | glycochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | chenodeoxycholate + glycine | - |
? | |
3.5.1.24 | glycochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum B14 | chenodeoxycholate + glycine | - |
? | |
3.5.1.24 | glycocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + glycine | - |
? | |
3.5.1.24 | glycocholic acid + H2O | - |
Lactiplantibacillus plantarum B14 | cholate + glycine | - |
? | |
3.5.1.24 | glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + glycine | - |
? | |
3.5.1.24 | glycodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum B14 | deoxycholate + glycine | - |
? | |
3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | chenodeoxycholate + taurine | - |
? | |
3.5.1.24 | taurochenodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum B14 | chenodeoxycholate + taurine | - |
? | |
3.5.1.24 | taurocholic acid + H2O | - |
Lactiplantibacillus plantarum | cholate + taurine | - |
? | |
3.5.1.24 | taurodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum | deoxycholate + taurine | - |
? | |
3.5.1.24 | taurodeoxycholic acid + H2O | - |
Lactiplantibacillus plantarum B14 | deoxycholate + taurine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.24 | bile salt hydrolase | - |
Lactiplantibacillus plantarum |
3.5.1.24 | BSH | - |
Lactiplantibacillus plantarum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 37 | - |
assay at | Lactiplantibacillus plantarum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.24 | 6 | - |
assay at | Lactiplantibacillus plantarum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.24 | evolution | microbial bile salt hydrolases (BSHs), a member of cholylglycine hydrolase (CGH) family. BSH is evolutionarily related to penicillin V acylase (PVA) which hydrolyses a penicillin V and is also a member of CGH family. five of the six amino acids, C2, R16, D19, N170, and R223, supposed to be responsible for catalytic activity of BSH enzyme, are strictly conserved in all CGH family members, N79 is partially conserved in the family | Lactiplantibacillus plantarum |
3.5.1.24 | additional information | residue N79 might be important for substrate binding and catalytic turnover of BSH. Structure homology modelling using the crystal structure of BSH from Enterococcus faecalis (PDB ID 4wl3.1.B) as template. The homology modelling and 3D structure of BSH indicates that N79 residue is located near the catalytic center and within the cavity of substrates accessory to the enzyme | Lactiplantibacillus plantarum |
3.5.1.24 | physiological function | microbial bile salt hydrolases (BSHs) catalyze the hydrolysis of glycine and taurine-linked bile salts in the small intestine of humans | Lactiplantibacillus plantarum |