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Literature summary extracted from

  • Lim, X.Y.; Pickford, R.; Don, A.S.
    Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry (2016), Methods Mol. Biol., 1376, 11-22 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.24 fumonisin B1
-
 Homo sapiens
2.3.1.297 fumonisin B1
-
 Homo sapiens
2.3.1.298 fumonisin B1
-
 Homo sapiens
2.3.1.299 fumonisin B1
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 cell membrane
-
 Homo sapiens
-
-
2.3.1.297 cell membrane
-
 Homo sapiens
-
-
2.3.1.298 cell membrane
-
 Homo sapiens
-
-
2.3.1.299 cell membrane
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.24 dihydrosphingosine + myristoyl-CoA Homo sapiens
-
 N-myristoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + palmitoyl-CoA Homo sapiens
-
 N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + palmitoyl-CoA Homo sapiens best substrate N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + stearoyl-CoA Homo sapiens
-
 N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 additional information Homo sapiens CERS4 transfers C18 and C20 fatty acids ?
-
 ?
2.3.1.24 additional information Homo sapiens CERS5 transfers C16 fatty acids, and potentially also C14 and C18 fatty acids ?
-
 ?
2.3.1.24 additional information Homo sapiens CERS6 transfers C14 and C16 fatty acids ?
-
 ?
2.3.1.24 sphingosine + eicosanoyl-CoA Homo sapiens
-
 N-eicosanoylsphingosine + CoA
-
 ?
2.3.1.297 additional information Homo sapiens CERS2 preferentially transfers very long chain fatty acids (C22-C26) ?
-
 ?
2.3.1.297 sphingosine + behenoyl-CoA Homo sapiens
-
 N-behenoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + cerotoyl-CoA Homo sapiens
-
 N-cerotoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + lignoceroyl-CoA Homo sapiens
-
 N-lignoceroylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + nervonoyl-CoA Homo sapiens
-
 N-nervonoylsphingosine + CoA
-
 ?
2.3.1.298 additional information Homo sapiens CERS3 transfers much longer chain fatty acids (C26, C28), forming highly hydrophobic ceramides that are a very important part of the water barrier function of skin ?
-
 ?
2.3.1.298 sphingosine + cerotoyl-CoA Homo sapiens
-
 N-cerotoylsphingosine + CoA
-
 ?
2.3.1.298 sphingosine + montanoyl-CoA Homo sapiens
-
 N-montanoylsphingosine + CoA
-
 ?
2.3.1.299 dihydrosphingosine + stearoyl-CoA Homo sapiens
-
 N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 additional information Homo sapiens CERS1 preferentially catalyzes the transfer of a C18:0 fatty acid, forming C18:0 dihydroceramide (d18:0/18:0 ceramide) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Homo sapiens Q6ZMG9
-
-
2.3.1.24 Homo sapiens Q8N5B7
-
-
2.3.1.24 Homo sapiens Q9HA82
-
-
2.3.1.297 Homo sapiens Q96G23
-
-
2.3.1.298 Homo sapiens Q8IU89
-
-
2.3.1.299 Homo sapiens P27544
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.298 skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 dihydrosphingosine + myristoyl-CoA
-
 Homo sapiens N-myristoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + palmitoyl-CoA
-
 Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + palmitoyl-CoA best substrate Homo sapiens N-palmitoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 dihydrosphingosine + stearoyl-CoA
-
 Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.24 additional information CERS4 transfers C18 and C20 fatty acids Homo sapiens ?
-
 ?
2.3.1.24 additional information CERS5 transfers C16 fatty acids, and potentially also C14 and C18 fatty acids Homo sapiens ?
-
 ?
2.3.1.24 additional information CERS6 transfers C14 and C16 fatty acids Homo sapiens ?
-
 ?
2.3.1.24 additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?
2.3.1.24 sphingosine + eicosanoyl-CoA
-
 Homo sapiens N-eicosanoylsphingosine + CoA
-
 ?
2.3.1.297 additional information CERS2 preferentially transfers very long chain fatty acids (C22-C26) Homo sapiens ?
-
 ?
2.3.1.297 additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?
2.3.1.297 sphingosine + behenoyl-CoA
-
 Homo sapiens N-behenoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + cerotoyl-CoA
-
 Homo sapiens N-cerotoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + lignoceroyl-CoA
-
 Homo sapiens N-lignoceroylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + nervonoyl-CoA
-
 Homo sapiens N-nervonoylsphingosine + CoA
-
 ?
2.3.1.298 additional information CERS3 transfers much longer chain fatty acids (C26, C28), forming highly hydrophobic ceramides that are a very important part of the water barrier function of skin Homo sapiens ?
-
 ?
2.3.1.298 additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?
2.3.1.298 sphingosine + cerotoyl-CoA
-
 Homo sapiens N-cerotoylsphingosine + CoA
-
 ?
2.3.1.298 sphingosine + montanoyl-CoA
-
 Homo sapiens N-montanoylsphingosine + CoA
-
 ?
2.3.1.299 dihydrosphingosine + stearoyl-CoA
-
 Homo sapiens N-stearoyldihydrosphingosine + CoA
-
 ?
2.3.1.299 additional information CERS1 preferentially catalyzes the transfer of a C18:0 fatty acid, forming C18:0 dihydroceramide (d18:0/18:0 ceramide) Homo sapiens ?
-
 ?
2.3.1.299 additional information CERS activity is assayed using LC-MS/MS for product quantification, crude extracts containing cell membranes are firstly prepared from tissues or cultured cells, reactions contain deuterated dihydrosphingosine (or sphingosine) and a fatty acid substrate linked to CoA (C16:0 to C24:0/24:1), detailed method descritpion and evaluation, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase 4
-
 Homo sapiens
2.3.1.24 ceramide synthase 5
-
 Homo sapiens
2.3.1.24 ceramide synthase 6
-
 Homo sapiens
2.3.1.24 CerS4
-
 Homo sapiens
2.3.1.24 CerS5
-
 Homo sapiens
2.3.1.24 CerS6
-
 Homo sapiens
2.3.1.297 ceramide synthase 2
-
 Homo sapiens
2.3.1.297 CerS2
-
 Homo sapiens
2.3.1.298 ceramide synthase 3
-
 Homo sapiens
2.3.1.298 CerS3
-
 Homo sapiens
2.3.1.299 ceramide synthase 1
-
 Homo sapiens
2.3.1.299 CerS1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 37
-
 assay at Homo sapiens
2.3.1.297 37
-
 assay at Homo sapiens
2.3.1.298 37
-
 assay at Homo sapiens
2.3.1.299 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.4
-
 assay at Homo sapiens
2.3.1.297 7.4
-
 assay at Homo sapiens
2.3.1.298 7.4
-
 assay at Homo sapiens
2.3.1.299 7.4
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.24 physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens
2.3.1.297 physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens
2.3.1.298 physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens
2.3.1.299 physiological function ceramides are synthesized by ceramide synthases through the addition of a variable length fatty acid to the amine group of a sphingoid base. In mammalian cells, the sphingoid base used for de novo ceramide synthesis is usually the C18:0 lipid dihydrosphingosine. Ceramide synthesis is catalyzed by a family of six ceramide synthases (CERS1-6), each of which preferentially transfers fatty acids of different lengths to the amine group of dihydrosphingosine Homo sapiens