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Literature summary extracted from

  • Linz, L.B.; Liu, S.; Chougule, N.P.; Bonning, B.C.
    In vitro evidence supports membrane alanyl aminopeptidase N as a receptor for a plant virus in the pea aphid vector (2015), J. Virol., 89, 11203-11212 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 recombinant expression of His-tagged enzyme APN in Spodoptera frugiperda SF9 cells using the baculovirus transfection system, recombinant expression of pea aphid APN in Escherichia coli for the production of polyclonal antibodies Acyrthosiphon pisum

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.2 additional information PEMV virions and a constructed peptide comprised of PEMV CP fused to a proline-rich hinge (-P-) and green fluorescent protein (CP-P-GFP) specifically bind to APN. Recombinant APN expression in Sf9 cells results in internalization of CP-P-GFP, visualized by confocal microscopy, signalizing a functional gut receptor Acyrthosiphon pisum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 brush border membrane vesicle
-
 Acyrthosiphon pisum
-
-
3.4.11.2 membrane
-
 Acyrthosiphon pisum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Zn2+ required Acyrthosiphon pisum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Acyrthosiphon pisum Q1W3E8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 recombinant His-tagged enzyme APN from Spodoptera frugiperda SF9 cells by nickel affinity chromatography Acyrthosiphon pisum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 gut
-
 Acyrthosiphon pisum
-

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 APN
-
 Acyrthosiphon pisum
3.4.11.2 membrane alanyl aminopeptidase N
-
 Acyrthosiphon pisum

General Information

EC Number General Information Comment Organism
3.4.11.2 physiological function membrane alanyl aminopeptidase N (APN) is a receptor for pea enation mosaic virus (PEMV) coat protein (CP) in the gut of the pea aphid, Acyrthosiphon pisum. PEMV virions specifically bind to APN. Aphid gut-derived brush border membrane vesicles, binding of recombinant CP-P-GFP construct compete with binding of GBP3.1, a peptide previously demonstrated to bind to APN in the aphid gut and to impede PEMV uptake into the hemocoel. GBP3.1 and PEMV bind to and compete for the same APN receptor Acyrthosiphon pisum