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Literature summary extracted from

  • Li, H.; Wang, X.; Ma, Y.; Yang, N.; Zhang, X.; Xu, Z.; Shi, J.
    Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia (2016), J. Mol. Catal. B, 128, 46-51 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.189 Cu2+
-
 Fusarium proliferatum
3.2.1.189 Fe2+
-
 Fusarium proliferatum
3.2.1.189 Fe3+
-
 Fusarium proliferatum
3.2.1.189 Mn2+
-
 Fusarium proliferatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.189 K+ slightly activating Fusarium proliferatum
3.2.1.189 Mg2+ slightly activating Fusarium proliferatum
3.2.1.189 additional information poor effects by Na+, Ca2+, Al3+, Ni2+, and Zn2+ Fusarium proliferatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.189 additional information Fusarium proliferatum strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A ?
-
 ?
3.2.1.189 additional information Fusarium proliferatum WX12 strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.189 Fusarium proliferatum
-
-
-
3.2.1.189 Fusarium proliferatum WX12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.189 native enzyme 8.51fold by ammonium sulfate fractionation, ultrafiltration, and anion exchange and hydrophobic interaction chromatography Fusarium proliferatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.189 additional information the optimum conditions are glucose 10 g/l, yeast 25 g/l, MgSO4 0.3 g/l, NaCl 1.16 g/l, KH2PO 42.72 g/l, pH 6.0, 30°C, resulting in 550 U/ml activity Fusarium proliferatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.189 additional information
-
 purified native enzyme shows 37.6 U/ml activity Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.189 3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + H2O i.e. sinodiosgenin or trillin Fusarium proliferatum D-glucose + L-rhamnose + L-arabinose + diosgenin
-
 ?
3.2.1.189 3-O-[alpha-L-Ara-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + H2O i.e. sinodiosgenin or trillin Fusarium proliferatum WX12 D-glucose + L-rhamnose + L-arabinose + diosgenin
-
 ?
3.2.1.189 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Fusarium proliferatum D-glucose + 2 L-rhamnose + diosgenin
-
 ?
3.2.1.189 3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 3 H2O
-
 Fusarium proliferatum WX12 D-glucose + 2 L-rhamnose + diosgenin
-
 ?
3.2.1.189 additional information strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A Fusarium proliferatum ?
-
 ?
3.2.1.189 additional information enzyme GiGly shows high substrate specificity for multi-3-O-glycosides of spirostanol saponins such as dioscin, trillin and polyphyllin VII, and is inactive toward substrates with terminal groups of rhamnosyl, glucosyl, galactosyl of ginsenoside Re and saikosaponin A. It can only hydrolyze glycosidic bonds at the C-3 position on steroidal saponins, which have similar structure with dioscin, and can be transformed into diosgenin. Substrate specificity, overview. GiGly is able tohydrolyze the terminal alpha-1,2-linked rhamnosyl residues, alpha-1,4-linked rhamnosyl residues, alpha-1,4-linked arabinosyl residues and beta-D-glucosyl residues at C-3 position.. The hydrolyzed substrates of GiGly have a double bond between carbons 5 and 6 position. By contrast, the non-substrates lack the said double bond and have substituents at carbon 4 position Fusarium proliferatum ?
-
 ?
3.2.1.189 additional information strain WX12 has ability to hydrolyze dioscin, trillin and polyphyllin VII into diosgenin as the final product. But it is inactive toward the terminal rhamnosyl, glucosyl and galactosyl of ginsenoside Re and saikosaponin A Fusarium proliferatum WX12 ?
-
 ?
3.2.1.189 additional information enzyme GiGly shows high substrate specificity for multi-3-O-glycosides of spirostanol saponins such as dioscin, trillin and polyphyllin VII, and is inactive toward substrates with terminal groups of rhamnosyl, glucosyl, galactosyl of ginsenoside Re and saikosaponin A. It can only hydrolyze glycosidic bonds at the C-3 position on steroidal saponins, which have similar structure with dioscin, and can be transformed into diosgenin. Substrate specificity, overview. GiGly is able tohydrolyze the terminal alpha-1,2-linked rhamnosyl residues, alpha-1,4-linked rhamnosyl residues, alpha-1,4-linked arabinosyl residues and beta-D-glucosyl residues at C-3 position.. The hydrolyzed substrates of GiGly have a double bond between carbons 5 and 6 position. By contrast, the non-substrates lack the said double bond and have substituents at carbon 4 position Fusarium proliferatum WX12 ?
-
 ?
3.2.1.189 polyphyllin VII + 4 H2O
-
 Fusarium proliferatum 3 L-rhamnose + D-glucose + (22zeta,25R)-9zeta-spirost-5-en-3beta,17-diol
-
 ?
3.2.1.189 polyphyllin VII + 4 H2O
-
 Fusarium proliferatum WX12 3 L-rhamnose + D-glucose + (22zeta,25R)-9zeta-spirost-5-en-3beta,17-diol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.189 monomer 1 * 45000, SDS-PAGE Fusarium proliferatum
3.2.1.189 More peptide mass fingerprinting, analysis through mass spectrometry and SDS-PAGE Fusarium proliferatum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.189 GiGly
-
 Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.189 50
-
-
 Fusarium proliferatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.189 4
-
 purified native enzyme GiGly conserved more than 70% of the original activity after 10 h of incubation at 4°C Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.189 8
-
-
 Fusarium proliferatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.189 7 8 purified enzyme GiGly, stable at pH 5.0-8.0 retaining 80% activity at pH 7.0 after 12 h Fusarium proliferatum