EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.1 | the aspartate product in the crystal structure of L-ASP exists in an unusual alpha-COOH protonation state. The crystal structures may represent intermediate steps rather than initial binding. The substrate's alpha-carboxyl may serve as a proton acceptor and activate one of the catalytic threonines during L-ASP's nucleophilic attack on the amide carbon | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.018 | - |
L-asparagine | K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 0.021 | - |
L-asparagine | K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 0.025 | - |
L-asparagine | K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 0.068 | - |
L-asparagine | K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 1.6 | - |
L-glutamine | K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Escherichia coli | P00805 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
3.5.1.1 | L-glutamine + H2O | reaction of EC 3.5.1.2 | Escherichia coli | L-glutamate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.1 | AnsB | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 2.2 | - |
L-glutamine | pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 48 | - |
L-asparagine | presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 60 | - |
L-asparagine | pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 67 | - |
L-asparagine | presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 72 | - |
L-asparagine | presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 710 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 1400 | - |
L-glutamine | using K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 2700 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 3300 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C | Escherichia coli | |
3.5.1.1 | 3400 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli |