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Literature summary extracted from

  • Arya, T.; Reddi, R.; Kishor, C.; Ganji, R.J.; Bhukya, S.; Gumpena, R.; McGowan, S.; Drag, M.; Addlagatta, A.
    Identification of the molecular basis of inhibitor selectivity between the human and streptococcal type I methionine aminopeptidases (2015), J. Med. Chem., 58, 2350-2357 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18
-
 Homo sapiens
3.4.11.18
-
 Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.18 F309M about 10% of wild-type activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 [amino(cyclohexyl)methyl]phosphonic acid compound selectively inhibits the Streptococcus pneumoniae MetAP in low micromolar range but not the human enzyme. Residue F309 in the human enzyme and M205 in the Streptococcus pneumoniae MetAP at the analogous position render them with different susceptibilities against the inhibitor Homo sapiens
3.4.11.18 [amino(cyclohexyl)methyl]phosphonic acid compound selectively inhibits the Streptococcus pneumoniae MetAP in low micromolar range but not the human enzyme. Residue F309 in the human enzyme and M205 in the Streptococcus pneumoniae MetAP at the analogous position render them with different susceptibilities against the inhibitor Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 0.386
-
 L-methionine 4-nitroanilide wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
3.4.11.18 0.4019
-
 L-methionine 4-nitroanilide mutant F309M, pH not specified in the publication, temperature not specified in the publication Homo sapiens
3.4.11.18 0.543
-
 L-methionine 4-nitroanilide pH not specified in the publication, temperature not specified in the publication Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Homo sapiens P53582
-
-
3.4.11.18 Streptococcus pneumoniae B2IQ22
-
-
3.4.11.18 Streptococcus pneumoniae CGSP14 B2IQ22
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 L-methionine 4-nitroanilide + H2O
-
 Homo sapiens L-methionine + 4-nitroaniline
-
 ?
3.4.11.18 L-methionine 4-nitroanilide + H2O
-
 Streptococcus pneumoniae L-methionine + 4-nitroaniline
-
 ?
3.4.11.18 L-methionine 4-nitroanilide + H2O
-
 Streptococcus pneumoniae CGSP14 L-methionine + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.18 MetAP1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 0.0033
-
 L-methionine 4-nitroanilide mutant F309M, pH not specified in the publication, temperature not specified in the publication Homo sapiens
3.4.11.18 0.005
-
 L-methionine 4-nitroanilide pH not specified in the publication, temperature not specified in the publication Streptococcus pneumoniae
3.4.11.18 0.0483
-
 L-methionine 4-nitroanilide wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.18 0.0079
-
 [amino(cyclohexyl)methyl]phosphonic acid pH not specified in the publication, temperature not specified in the publication Streptococcus pneumoniae
3.4.11.18 0.5
-
 [amino(cyclohexyl)methyl]phosphonic acid IC50 value above 0.5 mM, pH not specified in the publication, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.18 10
-
 L-methionine 4-nitroanilide pH not specified in the publication, temperature not specified in the publication Streptococcus pneumoniae
3.4.11.18 10
-
 L-methionine 4-nitroanilide mutant F309M, pH not specified in the publication, temperature not specified in the publication Homo sapiens
3.4.11.18 128
-
 L-methionine 4-nitroanilide wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens