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Literature summary extracted from

  • Huang, F.T.; Han, Y.B.; Feng, Y.; Yang, G.Y.
    A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production (2015), J. Lipid Res., 56, 1836-1842 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.69 sodium cholate activates at 0.07-0.7% w/v, loss of about 75% activation potency in absence of Ca2+ Shewanella algae
3.5.1.69 taurodeoxycholate hydrate TDC, activates at 0.07-0.7% w/v, TDC-based enhancement of hydrolysis requires the presence of Ca2+. In the absence of Ca2+, GM1 hydrolysis is only 2.9%, 14fold lower than the same case of Triton X-100 Shewanella algae
3.5.1.69 Triton X-100 activates at 0.07-0.7% w/v, loss of about 50% activation potency in absence of Ca2+ Shewanella algae
3.5.1.69 Tween 80 activates 0.07-0.7% w/v, loss of about 65% activation potency in absence of Ca2+ Shewanella algae

Application

EC Number Application Comment Organism
3.5.1.69 synthesis sphingolipid ceramide N-deacylase is used for lyso-glycosphingolipid production. Application to large-scale preparation of gamglioside lyso-GM1, overview Shewanella algae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.69 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Shewanella algae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.69 Ca2+ required, activtes. GM1 hydrolysis increases with increasing Ca 2+ concentration, compared with 40.7% yield in the absence of Ca2+, the addition of 100 mM Ca2+ enhances the yield to 81.6%. Best at 50-100 mM Shewanella algae
3.5.1.69 Co2+ activates, but slightly less effectve compared to Ca2+, at 100 mM Shewanella algae
3.5.1.69 Mg2+ activates, but less effectve compared to Ca2+, at 100 mM Shewanella algae
3.5.1.69 Mn2+ activates similar to Ca2+, at 100 mM Shewanella algae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.69 Shewanella algae Q8RTY6
-
-
3.5.1.69 Shewanella algae G8 Q8RTY6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.69 recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) by nickel affinity chromatography Shewanella algae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.69 galactosylceramide + H2O
-
 Shewanella algae ?
-
 ?
3.5.1.69 ganglioside GM1 + H2O
-
 Shewanella algae ?
-
 ?
3.5.1.69 ganglioside GM1 + H2O
-
 Shewanella algae G8 ?
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Shewanella algae ?
-
 ?
3.5.1.69 ganglioside GM3 + H2O
-
 Shewanella algae G8 ?
-
 ?
3.5.1.69 glucosylceramide + H2O
-
 Shewanella algae ?
-
 ?
3.5.1.69 glucosylceramide + H2O
-
 Shewanella algae G8 ?
-
 ?
3.5.1.69 additional information assay method optimization and evaluation, overview Shewanella algae ?
-
 ?
3.5.1.69 additional information assay method optimization and evaluation, overview Shewanella algae G8 ?
-
 ?
3.5.1.69 sphingomyelin + H2O
-
 Shewanella algae ?
-
 ?
3.5.1.69 sphingomyelin + H2O
-
 Shewanella algae G8 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.69 SA_SCD
-
 Shewanella algae
3.5.1.69 sphingolipid ceramide N-deacylase
-
 Shewanella algae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.69 37
-
 assay at Shewanella algae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.69 5.8
-
 assay at Shewanella algae

General Information

EC Number General Information Comment Organism
3.5.1.69 additional information development of a facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production Shewanella algae