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Literature summary extracted from
- Characterization of human S-adenosyl-homocysteine hydrolase in vitro and identification of its potential inhibitors (2017), J. Enzyme Inhib. Med. Chem., 32, 1209-1215 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | drug development | because of the prominent role of SAM-dependent transmethylation in capped methylated structure production at the 5'-terminus of viral mRNA, SAHH inhibitors could be developed to be broad-spectrum antiviral agents | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | gene sahH, codon-optimized gene, recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris under control of the strong AOX1 promoter | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | (1R,2S,3R)-3-(4-amino-7-fluoro-1H-imidazo[4,5-c]pyridin-1-yl)cyclopentane-1,2-diol | CAS:1214735-71-3 | Homo sapiens |  |
| 3.13.2.1 | (2E)-3-(4-chlorophenyl)prop-2-enoic acid | - |
Homo sapiens | |
| 3.13.2.1 | (2E)-3-(4-methoxyphenyl)prop-2-enoic acid | - |
Homo sapiens | |
| 3.13.2.1 | 1-(bromomethyl)-2,4-difluorobenzene | - |
Homo sapiens | |
| 3.13.2.1 | 1H-benzotriazol-1-ol | - |
Homo sapiens | |
| 3.13.2.1 | 4-(3-hydroxyprop-1-en-1-yl)-2-methoxyphenol | coniferyl alcohol, molecular docking studies show that coniferyl alcohol is well docked into the active cavity of SAHH. Several H-bonds are formed between them, which stabilize coniferyl alcohol in the active site of rSAHH with a proper conformation | Homo sapiens | |
| 3.13.2.1 | 4-chloro-1,3-dihydroxyphenol | - |
Homo sapiens | |
| 3.13.2.1 | 4-chlorobenzene-1-carboperoxoic acid | - |
Homo sapiens | |
| 3.13.2.1 | 4-fluorobenzoic acid | - |
Homo sapiens | |
| 3.13.2.1 | 4-methoxyaniline | - |
Homo sapiens | |
| 3.13.2.1 | 4-methoxybenzaldehyde | i.e. anisaldehyde | Homo sapiens | |
| 3.13.2.1 | 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol | - |
Homo sapiens | |
| 3.13.2.1 | ethyl 4-aminobenzoate | - |
Homo sapiens | |
| 3.13.2.1 | methyl (2E)-3-[4-(bromomethyl)phenyl]prop-2-enoate | - |
Homo sapiens | |
| 3.13.2.1 | additional information | molecular docking study and simulations | Homo sapiens | |
| 3.13.2.1 | neplanocin A | NepA, a natural product that is cytotoxic upon long term exposure and shows adenosine deaminase and adenosine kinase activity | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | additional information | - |
additional information | Michaelis-Menten kinetics, double-reciprocal form of the Michaelis-Menten equation | Homo sapiens | |
| 3.13.2.1 | 0.0218 | - |
S-adenosyl-L-homocysteine | pH 6.5, 41°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Homo sapiens | - |
L-homocysteine + adenosine | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Homo sapiens | P23526 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | recombinant His6-tagged enzyme from Pichia pastoris by nickel affinity chromatography | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Homo sapiens | L-homocysteine + adenosine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | ? | x * 47500, recombinant His-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | S-adenosyl-homocysteine hydrolase | - |
Homo sapiens |
| 3.13.2.1 | SAHH | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 41 | - |
recombinant His-tagged enzyme | Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 25 | 49 | activity range, profile overview | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 6.5 | - |
recombinant His-tagged enzyme | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 4.5 | 10.5 | activity range, profile overview | Homo sapiens |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.0000015 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | neplanocin A | |
| 3.13.2.1 | 0.000034 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-[(1E)-3-hydroxyprop-1-en-1-yl]-2-methoxyphenol | |
| 3.13.2.1 | 0.00004 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | (1R,2S,3R)-3-(4-amino-7-fluoro-1H-imidazo[4,5-c]pyridin-1-yl)cyclopentane-1,2-diol | |
| 3.13.2.1 | 0.000565 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-chlorobenzene-1-carboperoxoic acid | |
| 3.13.2.1 | 0.000571 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | (2E)-3-(4-chlorophenyl)prop-2-enoic acid | |
| 3.13.2.1 | 0.000679 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-fluorobenzoic acid | |
| 3.13.2.1 | 0.000814 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | methyl (2E)-3-[4-(bromomethyl)phenyl]prop-2-enoate | |
| 3.13.2.1 | 0.000899 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-methoxyaniline | |
| 3.13.2.1 | 0.000913 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 1-(bromomethyl)-2,4-difluorobenzene | |
| 3.13.2.1 | 0.000986 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | (2E)-3-(4-methoxyphenyl)prop-2-enoic acid | |
| 3.13.2.1 | 0.00111 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-chloro-1,3-dihydroxyphenol | |
| 3.13.2.1 | 0.001245 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 1H-benzotriazol-1-ol | |
| 3.13.2.1 | 0.001399 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | ethyl 4-aminobenzoate | |
| 3.13.2.1 | 0.002131 | - |
pH 6.5, 41°C, recombinant enzyme | Homo sapiens | 4-methoxybenzaldehyde | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | malfunction | the inhibition of SAHH can lead to intracellular SAH accumulation, which triggers the negative feedback inhibition to suppress the S-adenosyl-L-methionine (SAM)-dependent transmethylation | Homo sapiens |
| 3.13.2.1 | metabolism | the enzyme is involved in the activated methyl cycle (AMC) of mammalian cells, overview | Homo sapiens |
| 3.13.2.1 | physiological function | SAHH plays a critical role in the mammalian methylation processes | Homo sapiens |
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