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Literature summary extracted from

  • Tang, X.L.; Lu, X.F.; Wu, Z.M.; Zheng, R.C.; Zheng, Y.G.
    Biocatalytic production of (S)-2-aminobutanamide by a novel D-aminopeptidase from Brucella sp. with high activity and enantioselectivity (2018), J. Biotechnol., 266, 20-26 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.19 synthesis enantioselective hydrolysis of 2-aminobutanamide by 10 g/l recombinant cells harboring Dap results in 51.8% conversion in 10 min and 99.8% e.e, of (S)-2-aminobutanamide Brucella sp. F23/97

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.19 expression in Escherichia coli Brucella sp. F23/97

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.19 15.1
-
 D-2-aminobutanamide pH 8.0, 35°C Brucella sp. F23/97

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.19 Brucella sp. F23/97
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.19 recombinant enzyme Brucella sp. F23/97

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.19 D-2-aminobutanamide + H2O racemic, preferred substrate Brucella sp. F23/97 D-aminobutanoic acid + NH3
-
 ?
3.4.11.19 D-alanine amide + H2O 28.3% of the activity with D-2-aminobutanamide Brucella sp. F23/97 D-alanine + NH3
-
 ?
3.4.11.19 D-serine amide + H2O
-
 Brucella sp. F23/97 D-serine + NH3
-
 ?
3.4.11.19 glycine amide + H2O 29.6% of the activity with D-2-aminobutanamide Brucella sp. F23/97 glycine + NH3
-
 ?
3.4.11.19 additional information D-valine amide, D-proline amide and D-leucine amide show less than 2% of hte activity with D-2-aminobutanamide. No activity with D-arginine amide, D-phenylalanine amide, or with L-amino acid amides Brucella sp. F23/97 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.19 DAP
-
 Brucella sp. F23/97

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.19 45
-
-
 Brucella sp. F23/97

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.19 40
-
 2 h, less than 30% residual activity Brucella sp. F23/97

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.19 8
-
-
 Brucella sp. F23/97

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.19 7 9 significant decrease in enzyme activity below pH 7 or above pH 9 Brucella sp. F23/97