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Literature summary extracted from
- Screening, identification, and characterization of alpha-xylosidase from a soil metagenome (2016), J. Biosci. Bioeng., 122, 393-399 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.177 | additional information | no effect on activity by 10 mM EDTA | uncultured microorganism |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.177 | gene meXyl31, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | uncultured microorganism |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.177 | Cu2+ | 94% inhibition at 5 mM | uncultured microorganism |  |
| 3.2.1.177 | DMSO | 4% inhibition at 10% and 13% at 25% | uncultured microorganism | |
| 3.2.1.177 | ethanol | 30% inhibition at 10% and 75% at 25% | uncultured microorganism | |
| 3.2.1.177 | Fe2+ | 24% inhibition at 5 mM | uncultured microorganism | |
| 3.2.1.177 | additional information | no effect on activity by 10 mM EDTA | uncultured microorganism | |
| 3.2.1.177 | Zn2+ | 85% inhibition at 5 mM | uncultured microorganism | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.177 | 2.39 | - |
4-nitrophenyl alpha-D-xylopyranoside | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
| 3.2.1.177 | 8 | 9 | Isoprimeverose | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.177 | additional information | poor effect on activity by 5 mM Mg2+, Mn2+ and Ca2+ | uncultured microorganism |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.177 | tamarind seed xyloglucan + H2O | uncultured microorganism | - |
D-xylose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.177 | uncultured microorganism | A0A169PW02 | isolated from a soil metagenomic library | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.177 | recombinant His6-tagged enzyme MeXyl31 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | uncultured microorganism |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 0.2 | - |
pH 5.5, 45°C, recombinant enzyme, substrate alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc | uncultured microorganism |
| 3.2.1.177 | 0.22 | - |
pH 5.5, 45°C, recombinant enzyme, substrate alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc | uncultured microorganism |
| 3.2.1.177 | 0.54 | - |
pH 5.5, 45°C, recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside | uncultured microorganism |
| 3.2.1.177 | 2 | - |
pH 5.5, 45°C, recombinant enzyme, substrate isoprimeverose | uncultured microorganism |
| 3.2.1.177 | 69.5 | - |
pH 5.5, 45°C, recombinant enzyme, substrate 4-nitrophenyl alpha-D-xylopyranoside | uncultured microorganism |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.177 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | low activity | uncultured microorganism | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.177 | 4-nitrophenyl alpha-D-xylopyranoside + H2O | best substrate | uncultured microorganism | 4-nitrophenol + D-xylose | - |
? | |
| 3.2.1.177 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O | low activity | uncultured microorganism | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + D-xylose | - |
? | |
| 3.2.1.177 | alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O | low activity | uncultured microorganism | ? | - |
? | |
| 3.2.1.177 | isoprimeverose + H2O | 6-O-(alpha-D-xylopyranosyl)-D-glucose or 6-O-alpha-D-xylopyranosyl-beta-D-glucopyranose, low activity | uncultured microorganism | D-xylose + D-glucose | - |
? | |
| 3.2.1.177 | additional information | substrate specificty, overview. The enzyme is not active with 0.16% tamarind seed xyloglucan, maltose, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-beta-L-fucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside | uncultured microorganism | ? | - |
? | |
| 3.2.1.177 | tamarind seed xyloglucan + H2O | - |
uncultured microorganism | D-xylose + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.177 | MeXyl31 | - |
uncultured microorganism |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 45 | - |
recombinant enzyme | uncultured microorganism |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.177 | 7.69 | - |
Isoprimeverose | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
| 3.2.1.177 | 154 | - |
4-nitrophenyl alpha-D-xylopyranoside | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.177 | 5.5 | - |
recombinant enzyme | uncultured microorganism |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.2.1.177 | evolution | the enzyme belongs to the glycosylhydrolase family 31, GH31 | uncultured microorganism |
| 3.2.1.177 | physiological function | alpha-xylosidase is important for the degradation of oligoxyloglucan saccharides | uncultured microorganism |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.177 | 0.086 | - |
Isoprimeverose | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
| 3.2.1.177 | 64.4 | - |
4-nitrophenyl alpha-D-xylopyranoside | pH 5.5, 45°C, recombinant enzyme | uncultured microorganism | |
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