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Literature summary extracted from
- Isolation of a putative virulence agent, cytotoxic serine-elastase, from a newly isolated Pseudomonas aeruginosa ZuhP13 (2019), J. Biosci., 44, 7 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.26 | 1,10-phenanthroline | slight inhibition | Pseudomonas aeruginosa |  |
| 3.4.24.26 | 2,2'-bipyridine | slight inhibition | Pseudomonas aeruginosa | |
| 3.4.24.26 | Aprotinin | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Ba2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Ca2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Cd2+ | 5 mM, decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Co2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Cu2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | EDTA | slight inhibition | Pseudomonas aeruginosa | |
| 3.4.24.26 | Elastatinal | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Fe3+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Hg2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Mg2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Mn2+ | 5 mM, slightly decreases activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | PMSF | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Soybean trypsin inhibitor | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | TLCK | markedly decreases enzymatic activity | Pseudomonas aeruginosa | |
| 3.4.24.26 | Zn2+ | slight inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.26 | additional information | - |
azocasein | kcat/Km: 1.3 mg/ml, pH 7.5, 40°C | Pseudomonas aeruginosa |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.26 | extracellular | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 48000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.26 | Pseudomonas aeruginosa | - |
- |
- |
| 3.4.24.26 | Pseudomonas aeruginosa ZuhP13 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.26 | - |
Pseudomonas aeruginosa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.26 | azocasein + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | azocasein + H2O | - |
Pseudomonas aeruginosa ZuhP13 | ? | - |
? | |
| 3.4.24.26 | additional information | the enzyme shows both hemolytic and hemorrhagic activities in vivo | Pseudomonas aeruginosa | ? | - |
? | |
| 3.4.24.26 | additional information | the enzyme shows both hemolytic and hemorrhagic activities in vivo | Pseudomonas aeruginosa ZuhP13 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.26 | ? | x * 48000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 40 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 30 | 50 | 30°C: about 75% of maximal activity, 50°C: about 85% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 50 | - |
half-life time: 297.7 min | Pseudomonas aeruginosa |
| 3.4.24.26 | 60 | - |
30 min, stable | Pseudomonas aeruginosa |
| 3.4.24.26 | 60 | - |
half-life time: 97.3 min | Pseudomonas aeruginosa |
| 3.4.24.26 | 61.3 | - |
midpoint temperature | Pseudomonas aeruginosa |
| 3.4.24.26 | 70 | - |
half-life time: 26.5 min | Pseudomonas aeruginosa |
| 3.4.24.26 | 80 | - |
half-life time: 0 min | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.26 | 1.27 | - |
azocasein | pH 7.5, 40°C | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 7.5 | - |
assay at | Pseudomonas aeruginosa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.26 | 6 | 9 | 1 h, stable | Pseudomonas aeruginosa |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.24.26 | Pseudomonas aeruginosa | - |
- |
5.6 |
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