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Literature summary extracted from

  • Cussiol, J.R.; Alegria, T.G.; Szweda, L.I.; Netto, L.E.
    Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity, lipoyl-dependent peroxidase (2010), J. Biol. Chem., 285, 21943-21950 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.28 0.01093
-
 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complexlipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 0.03851
-
 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 0.04446
-
 Lipoamide pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 0.0451
-
 tert-butyl hydroperoxide pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 41.72
-
 H2O2 pH 7.4, 37°C Xylella fastidiosa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.28 Xylella fastidiosa Q9PCF4
-
-
1.11.1.28 Xylella fastidiosa 9a5c Q9PCF4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.28
-
 Xylella fastidiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.28 lipoamide + H2O2
-
 Xylella fastidiosa ? + H2O
-
 ?
1.11.1.28 lipoamide + H2O2
-
 Xylella fastidiosa 9a5c ? + H2O
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Xylella fastidiosa [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Xylella fastidiosa 9a5c [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Xylella fastidiosa [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Xylella fastidiosa 9a5c [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complexlipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Xylella fastidiosa [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complexlipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Xylella fastidiosa 9a5c [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Xylella fastidiosa [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O
-
 ?
1.11.1.28 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Xylella fastidiosa 9a5c [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complex]-N6-lipoyl-L-lysine + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.28 Ohr
-
 Xylella fastidiosa
1.11.1.28 organic hydroperoxide resistance protein
-
 Xylella fastidiosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.28 37
-
 assay at Xylella fastidiosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.28 9.5
-
 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complexlipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 9.53
-
 H2O2 pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 17.95
-
 tert-butyl hydroperoxide pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 17.95
-
 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 90.9
-
 Lipoamide pH 7.4, 37°C Xylella fastidiosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.28 7.4
-
 assay at Xylella fastidiosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.28 0.23
-
 H2O2 pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 460
-
 [dihydrolipoamide acyltransferase, subunit E2 from alpha-ketoglutarate dehydrogenase complex]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 870
-
 [dihydrolipoamide acyltransferase, subunit E2 from pyruvate dehydrogenase complexlipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 2060
-
 tert-butyl hydroperoxide pH 7.4, 37°C Xylella fastidiosa
1.11.1.28 2060
-
 Lipoamide pH 7.4, 37°C Xylella fastidiosa