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Literature summary extracted from

  • Hillas, P.J.; del Alba, F.S.; Oyarzabal, J.; Wilks, A.; Ortiz De Montellano, P.R.
    The AhpC and AhpD antioxidant defense system of Mycobacterium tuberculosis (2000), J. Biol. Chem., 275, 18801-18809 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.28 medicine AhpCis a critical element of the antioxidant defense system of Mycobacterium tuberculosis and may be suitable target for the development of novel anti-tuberculosis strategies Mycobacterium tuberculosis
1.11.1.28 medicine AhpD is a critical element of the antioxidant defense system of Mycobacterium tuberculosis and may be suitable target for the development of novel anti-tuberculosis strategies Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.28
-
 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.28 C174S mutant enzyme retains about 10% of the wild-type activity Mycobacterium tuberculosis
1.11.1.28 C176S mutant enzyme retains about 10% of the wild-type activity Mycobacterium tuberculosis
1.11.1.28 C61S inactive mutant enzyme Mycobacterium tuberculosis
1.11.1.28 Cys129 mutant enzyme shows no activity with cumene hydroperoxide Mycobacterium tuberculosis
1.11.1.28 Cys132 mutant enzyme retains about 5% of the wild-type activity with cumene hydroperoxide Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.28 0.052
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.091
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.132
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.15
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.23
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.55
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.86
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 1.1
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 1.5
-
 H2O2 pH 7.0, 25°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.28 Mycobacterium tuberculosis P9WQB5 alkyl hydroperoxide reductase D (ahpD)
-
1.11.1.28 Mycobacterium tuberculosis P9WQB7 alkyl hydroperoxide reductase C (ahpC)
-
1.11.1.28 Mycobacterium tuberculosis ATCC 25618 P9WQB5 alkyl hydroperoxide reductase D (ahpD)
-
1.11.1.28 Mycobacterium tuberculosis ATCC 25618 P9WQB7 alkyl hydroperoxide reductase C (ahpC)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.28
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + 7alpha-hydroxy-3beta-hydroxycholest-6-ene
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + 7alpha-hydroxy-3beta-hydroxycholest-6-ene
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cholesterol hydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cholesterol hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cholesterol hydroperoxide
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cholesterol hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide best substrate for AhpD Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide best substrate for AhpD Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + cumene hydroperoxide
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + cumene hydroxide
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + H2O2
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + 2 H2O
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + oleic acid hydroperoxide poor substrate Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + oleic acid
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + oleic acid hydroperoxide poor substrate Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + oleic acid
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + tert-butyl hydroperoxide
-
 Mycobacterium tuberculosis ATCC 25618 a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + tert-butyl alcohol
-
 ?
1.11.1.28 a [lipoyl-carrier protein]-N6-[(R)-dihydrolipoyl]-L-lysine + trans-pinocarveylhydroperoxide
-
 Mycobacterium tuberculosis a [lipoyl-carrier protein]-N6-lipoyl-L-lysine + H2O + trans-pinocarveyl alcohol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.28 peroxiredoxin AhpC
-
 Mycobacterium tuberculosis
1.11.1.28 peroxiredoxin AhpD
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.28 0.013
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.015
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.033
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.035
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.06
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.067
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.085
-
 H2O2 pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.088
-
 H2O2 pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.147
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.178
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.11.1.28 physiological function AhpC is a critical element of the antioxidant defense system of Mycobacterium tuberculosis Mycobacterium tuberculosis
1.11.1.28 physiological function AhpD is a critical element of the antioxidant defense system of Mycobacterium tuberculosis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.28 0.017
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.032
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.056
-
 H2O2 pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.059
-
 H2O2 pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.06
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.087
-
 trans-pinocarveylhydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 0.26
-
 tert-butyl hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 1.2
-
 cumene hydroperoxide pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 1.35
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis
1.11.1.28 1.62
-
 7alpha-hydroperoxy-3beta-hydroxycholest-6-ene pH 7.0, 25°C Mycobacterium tuberculosis