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Literature summary extracted from

  • Onaca, C.; Kieninger, M.; Engesser, K.; Altenbuchner, J.
    Degradation of alkyl methyl ketones by Pseudomonas veronii MEK700 (2007), J. Bacteriol., 189, 3759-3767 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.113 expression in Escherichia coli Pseudomonas veronii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.113 ethyl acetate + H2O Pseudomonas veronii
-
 acetate + ethanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.113 Pseudomonas veronii Q0MRG5
-
-
3.1.1.113 Pseudomonas veronii MEK700 Q0MRG5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.113 3.4
-
 substrate 4-nitrophenyl butanoate, pH 7.0, 30°C Pseudomonas veronii
3.1.1.113 77
-
 substrate propyl acetate, pH 7.0, 30°C Pseudomonas veronii
3.1.1.113 78
-
 substrate methyl acetate, pH 7.0, 30°C Pseudomonas veronii
3.1.1.113 217
-
 substrate 4-nitrophenyl acetate, pH 7.0, 30°C Pseudomonas veronii
3.1.1.113 230
-
 substrate ethyl acetate, pH 7.0, 30°C Pseudomonas veronii
3.1.1.113 288
-
 substrate vinyl acetate, pH 7.0, 30°C Pseudomonas veronii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.113 4-nitrophenyl acetate + H2O
-
 Pseudomonas veronii 4-nitrophenol + acetate
-
 ?
3.1.1.113 4-nitrophenyl acetate + H2O
-
 Pseudomonas veronii MEK700 4-nitrophenol + acetate
-
 ?
3.1.1.113 4-nitrophenyl butanoate + H2O
-
 Pseudomonas veronii 4-nitrophenol + butanoate
-
 ?
3.1.1.113 4-nitrophenyl butanoate + H2O
-
 Pseudomonas veronii MEK700 4-nitrophenol + butanoate
-
 ?
3.1.1.113 ethyl acetate + H2O
-
 Pseudomonas veronii ethanol + acetate
-
 ?
3.1.1.113 ethyl acetate + H2O
-
 Pseudomonas veronii MEK700 ethanol + acetate
-
 ?
3.1.1.113 ethyl acetate + H2O
-
 Pseudomonas veronii acetate + ethanol
-
 ?
3.1.1.113 methyl acetate + H2O
-
 Pseudomonas veronii methanol + acetate
-
 ?
3.1.1.113 methyl acetate + H2O
-
 Pseudomonas veronii MEK700 methanol + acetate
-
 ?
3.1.1.113 propyl acetate + H2O
-
 Pseudomonas veronii propanol + acetate
-
 ?
3.1.1.113 propyl acetate + H2O
-
 Pseudomonas veronii MEK700 propanol + acetate
-
 ?
3.1.1.113 vinyl acetate + H2O
-
 Pseudomonas veronii ethenol + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.113 mekB
-
 Pseudomonas veronii

General Information

EC Number General Information Comment Organism
3.1.1.113 physiological function strain Pseudomonas veronii MEK700 is able to grow on 2-butanone and 2-hexanol. Inactivation of monooxygenase MekA and MekB abolishes growth . All three genes MekA, MekB and transcriptional activator MekR are required for growth of Pseudomonas veronii MEK700 on 2-butanone and 2-hexanol Pseudomonas veronii