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Literature summary extracted from

  • Reynolds, K.A.; Wang, P.; Fox, K.M.; Speedie, M.K.; Lam, Y.; Floss, H.G.
    Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus (1992), J. Bacteriol., 174, 3850-3854 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.120 iodoacetamide 1 mM, 52% inhibition Streptomyces collinus
1.3.1.120 N-ethylmaleimide 1 mM, 100% inhibition Streptomyces collinus
1.3.1.120 NADP+ cofactor inhibition Streptomyces collinus
1.3.1.120 p-chloromercuribenzoate 0.002 mM, 65% inhibition Streptomyces collinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.120 0.0015
-
 NADPH pH 7.4, 30°C Streptomyces collinus
1.3.1.120 0.025
-
 cyclohexane-1-carbonyl-CoA pH 7.4, 30°C Streptomyces collinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.120 Ca2+ 1 mM, 88% inhibition Streptomyces collinus
1.3.1.120 Co2+ 1 mM, 80% inhibition Streptomyces collinus
1.3.1.120 Mg2+ 1 mM, 58% inhibition Streptomyces collinus
1.3.1.120 Mn2+ 1 mM, 85% inhibition Streptomyces collinus
1.3.1.120 Zn2+ 1 mM, 100% inhibition Streptomyces collinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.120 75000
-
 gel filtration Streptomyces collinus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.120 Streptomyces collinus P95727
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.120
-
 Streptomyces collinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.120 cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+
-
 Streptomyces collinus cyclohexane-1-carbonyl-CoA + NADP+
-
 r
1.3.1.120 cyclohexa-1,3-diene-1-carbonyl-CoA + NADP+ 13% of activity Streptomyces collinus cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+
-
 ?
1.3.1.120 cyclohexane-1-carbonyl-CoA + NADP+
-
 Streptomyces collinus cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.1.120 dimer 2 * 36000, SDS-PAGE Streptomyces collinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.120 30
-
-
 Streptomyces collinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.120 7.5
-
-
 Streptomyces collinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.120 additional information no activity found with NADH Streptomyces collinus
1.3.1.120 NADPH
-
 Streptomyces collinus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.120 0.004
-
 NADP+ pH 7.4, 30°C Streptomyces collinus

General Information

EC Number General Information Comment Organism
1.3.1.120 physiological function the 1-cyclohexenylcarbonyl-CoA reductase catalyzes the final reductive step in the conversion of shikimic acid into asatrienin A Streptomyces collinus